Assessing the toxicity of TBBPA and HBCD by zebrafish embryo toxicity assay and biomarker analysis

Authors

  • Jun Hu,

    Corresponding author
    1. School of the Environment, Nanjing University; State Key Laboratory of Pollution Control and Resource Reuse, Nanjing, 210093, People's Republic of China
    • School of the Environment, Nanjing University; State Key Laboratory of Pollution Control and Resource Reuse, Nanjing, 210093, People's Republic of China
    Search for more papers by this author
  • Yong Liang,

    1. School of Medicine, Jianghan University, Hubei Province 430056, People's Republic of China
    Search for more papers by this author
  • Minjie Chen,

    1. School of Medicine, Jianghan University, Hubei Province 430056, People's Republic of China
    Search for more papers by this author
  • Xiaorong Wang

    1. School of the Environment, Nanjing University; State Key Laboratory of Pollution Control and Resource Reuse, Nanjing, 210093, People's Republic of China
    Search for more papers by this author

Abstract

Tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCD) are two of the most widely used brominated flame retardants (BFRs). The biological toxicity effect of TBBPA and HBCD was studied by means of zebrafish embryo toxicity assays in combination with three biomarkers, including superoxide dismutase (SOD), lipid peroxidation, (LPO), and heat shock protein (Hsp70). The standard zebrafish embryo assay showed that high concentrations of TBBPA (≥0.75 mg/L) can cause lethality or malformation. For HBCD within the concentration range (0.002–10 mg/L), no endpoint was observed. Furthermore, SOD activities of zebrafish embryos exposed to TBBPA were increased with the increasing concentrations. SOD activities in the group treated by HBCD showed an increase followed by a decline. Regardless of TBBPA or HBCD, LPO were increased along with the increase of the concentration. The change pattern of Hsp70 levels was the same with LPO. All these results showed that TBBPA and HBCD could cause oxidative stress and Hsp70 overexpression, inducing acute toxicity to zebrafish embryo in a short-term exposure. The study also indicates that the zebrafish embryo assay in combination with the biomarkers is effective in aquatic environmental toxicology and risk assessment. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.

Ancillary