Assessing the toxicity of herbicide isoproturon on Aporrectodea caliginosa (Oligochaeta) and its fate in soil ecosystem
Article first published online: 29 SEP 2008
Copyright © 2008 Wiley Periodicals, Inc.
Volume 24, Issue 4, pages 396–403, August 2009
How to Cite
Mosleh, Y. Y. I. (2009), Assessing the toxicity of herbicide isoproturon on Aporrectodea caliginosa (Oligochaeta) and its fate in soil ecosystem. Environ. Toxicol., 24: 396–403. doi: 10.1002/tox.20437
- Issue published online: 1 JUL 2009
- Article first published online: 29 SEP 2008
- Manuscript Accepted: 19 JUL 2008
- Manuscript Revised: 6 JUL 2008
- Manuscript Received: 20 JUL 2007
- earthworm (A. caliginosa);
- total soluble protein;
This study was conducted to determine the residues of isoproturon and its metabolites, 1-(4-isopropylphenyl)-3-methylurea, 1-(4-isopropylphenyl) urea, and 4-isopropylanilin in soil and mature earthworms under laboratory conditions. Mature earthworms (Aporrectodea caliginosa) were exposed for various durations (7, 15, 30, and 60 days) to soils contaminated with isoproturon concentrations (2, 4, 6, 8, and 10 mg kg−1 soil). The decrease in isoproturon concentration in the soil was inversely correlated to it's initial concentration. The highest concentration detected for isoproturon in earthworms was observed during the first 15 days and decreased thereafter. Acute toxicity of isoproturon was investigated; total soluble protein content and glycogen of the worms were evaluated. Levels of these parameters were related to isoproturon concentration in soil and earthworms. No lethal effect of isoproturon was observed even at the concentration of 1200 mg kg−1 soil after 60 days of exposure. A reduction of total soluble protein was observed in all treated worms (maximum 59.54%). This study suggests the use of the total soluble protein content and glycogen of earthworms as biomarkers of exposure to isoproturon. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.