Amifostine protection against induced DNA damage in γ-irradiated Escherichia coli cells depend on recN DNA repair gene product activity

  1. Eliseo Almeida1,
  2. Jorge Luis Fuentes2,*,
  3. Elizabeth Cuetara3,
  4. Elio Prieto4,
  5. Montserrat Llagostera5,6

Article first published online: 27 APR 2009

DOI: 10.1002/tox.20483

Issue

Environmental Toxicology

Environmental Toxicology

Volume 25, Issue 2, pages 130–136, April 2010

Additional Information

How to Cite

Almeida, E., Fuentes, J. L., Cuetara, E., Prieto, E. and Llagostera, M. (2010), Amifostine protection against induced DNA damage in γ-irradiated Escherichia coli cells depend on recN DNA repair gene product activity. Environ. Toxicol., 25: 130–136. doi: 10.1002/tox.20483

Author Information

  1. 1

    Departamento de Radiobiología, Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Apartado Postal 6122, Calle 30, # 502, e/ 5ta y 7ma, Miramar, Playa, Ciudad de la Habana, Cuba

  2. 2

    Laboratorio de Microbiología y Mutagénesis Ambiental, Escuela de Biología, Facultad de Ciencias, Universidad Industrial de Santander, Apartado Aéreo 678, Ciudad Universitaria, Carrera 27-Calle 9, Bucaramanga, Colombia

  3. 3

    Laboratorio de Farmacología Clínica y Experimental, Instituto Nacional de Oncología y Radiobiología, Calle 29 e/ F y G, Vedado, Ciudad de la Habana, Cuba

  4. 4

    Centro de Altos Estudios en Ciencias de la Salud, Universidad Abierta Interamericana, Ave Montes de Oca, No. 745, CP. 1270, Buenos Aires, Argentina

  5. 5

    Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Edifici C, 08193 Cerdanyola del Vallès, Bellaterra, Spain

  6. 6

    Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona-Institut de Recerca i Tecnologia Agroalimentària (UAB-IRTA), Bellaterra, Barcelona 08193, Spain

*Laboratorio de Microbiología y Mutagénesis Ambiental, Escuela de Biología, Facultad de Ciencias, Universidad Industrial de Santander, Apartado Aéreo 678, Ciudad Universitaria, Carrera 27-Calle 9, Bucaramanga, Colombia

Publication History

  1. Issue published online: 5 MAR 2010
  2. Article first published online: 27 APR 2009
  3. Manuscript Accepted: 17 JAN 2009
  4. Manuscript Revised: 15 JAN 2009
  5. Manuscript Received: 26 MAR 2008

Funded by

  • Cuban Nuclear Agency of the Ministry of Science, Technology, and Environment. Grant Number: PRN/7-1/3-2002

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Keywords:

  • amifostine;
  • Escherichia coli;
  • γ-rays;
  • recN gene;
  • SOS response

Abstract

Amifostine is the most effective radioprotector known and the only one accepted for clinical use in cancer radiotherapy. In this work, the antigenotoxic effect of amifostine against γ-rays was studied in Escherichia coli cells deficient in DNA damage repair activities. Assays of irradiated cells treated with amifostine showed that the drug reduced the genotoxicity induced by radiation in E. coli wild-type genotypes and in uvr, recF, recB, recB-recC-recF mutant strains, but not in recN defective cells. Thus, the mechanism of DNA protection by amifostine against γ-radiation-induced genotoxicity appears to involve participation of the RecN protein that facilitates repair of DNA double-strand breaks. The results are discussed in relation to amifostine's chemopreventive potential. © 2009 Wiley Periodicals, Inc. Environ Toxicol, 2010.

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