Chronic contamination assessment integrating biomarkers' responses in transplanted mussels—A seasonal monitoring

Authors

  • C. D. S. Pereira,

    Corresponding author
    1. Universidade Santa Cecília, Laboratório de Ecotoxicologia, Oswaldo Cruz 266, 11045-907 Santos, SP, Brazil
    2. Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil
    3. Departamento de Química Física, Facultad de Ciencias del Mar y Ambientales, Polígono Río San Pedro, s/n, 11510 Puerto Real, Cádiz, Spain
    • Universidade Santa Cecília, Laboratório de Ecotoxicologia, Oswaldo Cruz 266, 11045-907 Santos, SP, Brazil
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  • M. L. Martín-Díaz,

    1. Departamento de Química Física, Facultad de Ciencias del Mar y Ambientales, Polígono Río San Pedro, s/n, 11510 Puerto Real, Cádiz, Spain
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  • M. G. M. Catharino,

    1. Instituto de Pesquisas Energéticas e Nuclerares, IPEN-CNEN/SP, CP 05508-000 São Paulo, SP, Brazil
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  • A. Cesar,

    1. Universidade Santa Cecília, Laboratório de Ecotoxicologia, Oswaldo Cruz 266, 11045-907 Santos, SP, Brazil
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  • R. B. Choueri,

    1. Universidade Santa Cecília, Laboratório de Ecotoxicologia, Oswaldo Cruz 266, 11045-907 Santos, SP, Brazil
    2. Departamento de Química Física, Facultad de Ciencias del Mar y Ambientales, Polígono Río San Pedro, s/n, 11510 Puerto Real, Cádiz, Spain
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  • S. Taniguchi,

    1. Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil
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  • D. M. S. Abessa,

    1. Universidade Estadual Paulista, CLP, Núcleo de Estudos em Poluição e Ecotoxicologia Aquática, Praça Infante Dom Henrique, s/n, 11330-900 São Paulo, SP, Brazil
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  • M. C. Bícego,

    1. Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil
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  • M. B. A. Vasconcellos,

    1. Instituto de Pesquisas Energéticas e Nuclerares, IPEN-CNEN/SP, CP 05508-000 São Paulo, SP, Brazil
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  • A. C. D. Bainy,

    1. Departamento de Bioquímica, CCB, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil
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  • E. C. P. M. Sousa,

    1. Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-900 São Paulo, SP, Brazil
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  • T. A. DelValls

    1. Departamento de Química Física, Facultad de Ciencias del Mar y Ambientales, Polígono Río San Pedro, s/n, 11510 Puerto Real, Cádiz, Spain
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  • This paper was presented at the 14th International Symposium on Toxicity Assessment held at Metz, France, on August 30 to September 4, 2009.

Abstract

This study aimed to provide the first biomonitoring integrating biomarkers and bioaccumulation data in São Paulo coast, Brazil and, for this purpose, a battery of biomarkers of defense mechanisms was analyzed and linked to contaminants' body burden in a weigh-of-evidence approach. The brown mussel Perna perna was selected to be transplanted from a farming area (Caraguatatuba) to four possibly polluted sites: Engenho D'Água, DTCS (Dutos e Terminais do Centro-Oeste de São Paulo) oil terminal (Sao Sebastiao zone), Palmas Island, and Itaipu (It; Santos Bay zone). After 3 months of exposure in each season, mussels were recollected and the cytochrome P4501A (CYP1A)- and CYP3A-like activities, glutathione-S-transferase and antioxidants enzymes (catalase, glutathione peroxidase, and glutathione reductase) were analyzed in gills. The concentrations of polycyclic aromatic hydrocarbons, linear alkylbenzenes, and nonessential metals (Cr, Cd, Pb, and Hg) in whole tissue were also analyzed and data were linked to biomarkers' responses by multivariate analysis (principal component analysis—factor analysis). A representation of estimated factor scores was performed to confirm the factor descriptions and to characterize the studied stations. Biomarkers exhibited most significant alterations all year long in mussels transplanted to It, located at Santos Bay zone, where bioaccumulation of organic and inorganic compounds was detected. This integrated approach using transplanted mussels showed satisfactory results, pointing out differences between sites, seasons, and critical areas, which could be related to land-based contaminants' sources. The influence of natural factors and other contaminants (e.g., pharmaceuticals) on biomarkers' responses are also discussed. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.

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