p,p'-DDE induces apoptosis and mRNA expression of apoptosis-associated genes in testes of pubertal rats
Article first published online: 7 MAR 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 28, Issue 1, pages 31–41, January 2013
How to Cite
Shi, Y.-Q., Li, H.-W., Wang, Y.-P., Liu, C.-J. and Yang, K.-D. (2013), p,p'-DDE induces apoptosis and mRNA expression of apoptosis-associated genes in testes of pubertal rats. Environ. Toxicol., 28: 31–41. doi: 10.1002/tox.20694
- Issue published online: 13 DEC 2012
- Article first published online: 7 MAR 2011
- Manuscript Accepted: 18 DEC 2010
- Manuscript Revised: 16 DEC 2010
- Manuscript Received: 3 SEP 2010
- National Natural Science Foundation of China. Grant Numbers: 30972436, 30671734
- mitochondria pathway;
- endoplasmic reticulum pathway;
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. To elucidate the mechanism underpinning the testicular effects of p,p'-DDE, we sought to investigate apoptotic effects and mRNA expression of apoptosis-associated genes in the testis of pubertal rats, including Fas, FasL, calpain-1, cytochrome c, Bax, Bcl-w, Bak, and caspase-3, -8, -9, -12. Animals were administered with different doses of p,p'-DDE (0, 20, 60, 100 mg/kg body weight) every other day by intraperitoneal injection for 10 days. The results indicated that p,p'-DDE exposure at over 20 mg/kg body weight showed the induction of apoptotic cell death. p,p'-DDE could induce decrease in SOD and GSH-Px activity of serum in 60 mg/kg body weight group. Significant elevations in the mRNA levels of Fas, FasL, calpain-1, cytochrome c, Bax, Bak, and caspase-3, -8, -9, -12 were observed in testis of rat treated with p,p'-DDE. Taken together, these results lead us to speculate that in vivo exposure to p,p'-DDE might induce testicular apoptosis in pubertal rats through the involvement of Fas/FasL, mitochondria and endoplasmic reticulum-mediated pathways. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2013.