In vitro toxicology of ambient particulate matter: Correlation of cellular effects with particle size and components
Article first published online: 7 MAR 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 28, Issue 2, pages 76–86, February 2013
How to Cite
Kroll, A., Gietl, J. K., Wiesmüller, G. A., Günsel, A., Wohlleben, W., Schnekenburger, J. and Klemm, O. (2013), In vitro toxicology of ambient particulate matter: Correlation of cellular effects with particle size and components. Environ. Toxicol., 28: 76–86. doi: 10.1002/tox.20699
- Issue published online: 12 JAN 2013
- Article first published online: 7 MAR 2011
- Manuscript Accepted: 27 DEC 2010
- Manuscript Received: 13 APR 2010
- Federal German Ministry of Education and Research (BMBF; NanoCare, Förderkennzeichen). Grant Number: 03X0021C
- particulate matter;
- reactive oxygen species;
- in vitro toxicity;
- size distribution
High concentrations of airborne particulate matter (PM) have been associated with increased rates of morbidity and mortality among exposed populations. Although certain components of PM were suggested to influence these effects, no clear-cut correlation was determined thus far. One of the possible modes of action is the induction of oxidative stress by inhaled PM triggering inflammatory responses. Therefore, the in vitro formation of reactive oxygen species (ROS) in three cell lines in the presence of five subfractions of PM10, collected in Münster, Germany was investigated. The PM components chloride, nitrate, ammonium, sulfate, 68 chemical elements, and endotoxin were quantified. The highest concentration of endotoxin was found in particles of 0.42–1.2 μm aerodynamic diameters, and therefore probably subject to long-range transport. Intracellular ROS formation in three well established mammalian cell lines (CaCo2, human; MDCK, canine; RAW264.7, mouse) only correlated positively with particle size. The two smallest PM size fractions provoked the highest rise in ROS. However, the latter did not correlate with the concentration of any PM components investigated. The smallest PM size fractions significantly dominated the number of particles. Therefore, the particle number may be most effective in inducing oxidative stress in vitro. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.