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cDNA microarray analysis of the gene expression of murine leukemia RAW 264.7 cells after exposure to propofol

Authors

  • Rick Sai-Chuen Wu,

    1. Department of Anesthesiology, China Medical University Hospital, Taichung 404, Taiwan
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  • Kuo-Ching Liu,

    1. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan
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  • Nou-Ying Tang,

    1. School of Chinese Medicine, China Medical University, Taichung 404, Taiwan
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  • Hsiung-Kwang Chung,

    1. Graduate Institute of Clinical Medical Science, China Medical University, Taichung 404, Taiwan
    2. Department of Otorhinolaryngology—Head and Neck Surgery, China Medical University Hospital, Taichung 404, Taiwan
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  • Siu-Wan Ip,

    1. Department of Nutrition, China Medical University, Taichung 404, Taiwan
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  • Jai-Sing Yang,

    Corresponding author
    1. Department of Pharmacology, China Medical University, Taichung 404, Taiwan
    • Department of Anesthesiology, China Medical University Hospital, Taichung 404, Taiwan
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  • Jing-Gung Chung

    Corresponding author
    1. Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan
    2. Department Biotechnology, Asia University, Taichung 413, Taiwan
    • Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan
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Department of Pharmacology, China Medical University, Taichung 404, TaiwanDepartment of Biological Science and Technology, China Medical University, Taichung 404, Taiwan. E-mail: jaising@mail.cmu.edu.tw jgchung@ mail.cmu.edu.tw or jaising@mail.cmu.edu.tw

Abstract

Propofol (2,6-diisopropylphenol) is the most extensively used general anesthetic-sedative agent and it is employed in clinical patients. It has been shown that propofol exhibits anticancer activities. However, there is no available information to address propofol-induced cytotoxic effects and affected gene expressions on murine leukemia cells. Therefore, we investigated the effects of propofol on the levels of protein and gene expression, which are associated with apoptotic death in mouse leukemia RAW 264.7 cells in vitro. Results indicated that propofol induced cell morphological changes, cytotoxicity, and induction of apoptosis in RAW 264.7 cells in vitro. Western blot analysis demonstrated that propofol promoted Fas, cytochrome c, caspase-9 and −3 active form and Bax levels, but inhibited Bcl-xl protein level which led to cell apoptosis. Furthermore, cDNA microarray assay indicated that propofol significantly enhanced 5 gene expressions (Gm4884; Gm10883; Lce1c; Lrg1; and LOC100045878) and significantly suppressed 26 gene expressions (Gm10679; Zfp617; LOC621831; LOC621831; Gm5929; Snord116; Gm3994; LOC380994; Gm5592; LOC380994; Gm4638; LOC280487; Gm4638; Tex24; A530064D06Rik; BC094916; EG668725; Gm189; Hist2h3c2; Gm8020; Snord115; Gm3079; Olfr198; Tdh; Snord115; and Olfr1249). Based on these observations, propofol-altered apoptosis-related proteins might result from induction of apoptotic gene expression and inhibition of cell growth gene expression, which finally led to apoptosis in a mouse leukemia cell line (RAW 264.7) in vitro. © 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 471–478, 2013.

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