In vivo examination of the genotoxicity of the urban air and surface soil pollutant, 3,6-dinitrobenzo[e]pyrene, with intraperitoneal and intratracheal administration

Authors

  • Tatsuya Kato,

    1. Laboratory of Food Hygiene, Graduate School of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan
    2. Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan
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  • Yukari Totsuka,

    1. Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan
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  • Tomohiro Hasei,

    1. Department of Public Health, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan
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  • Tetsushi Watanabe,

    1. Department of Public Health, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan
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  • Keiji Wakabayashi,

    1. Laboratory of Food Hygiene, Graduate School of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan
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  • Naohide Kinae,

    1. Laboratory of Food Hygiene, Graduate School of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan
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  • Shuichi Masuda

    Corresponding author
    • Laboratory of Food Hygiene, Graduate School of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan
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S. Masuda. E-mail: masudas@u-shizuoka-ken.ac.jp

Abstract

3,6-Dinitrobenzo[e]pyrene (3,6-DNBeP) was identified as a new potent mutagen toward Salmonella strains in surface soil and airborne particles. Because data of in vivo examination of the genotoxicity of 3,6-DNBeP are limited, micronucleus test was performed in peripheral blood and bone marrow, and comet assay in the lungs of mice treated with 3,6-DNBeP. In male ICR mice intraperitoneally (i.p.) injected with 3,6-DNBeP, the frequency of micronuclated polychromatic erythrocytes (MNPCEs) was increased in the peripheral blood and bone marrow after 24 h in a dose-dependent manner. Compared to controls, the highest dose of 3,6-DNBeP (40 mg/kg B.W.) induced 7.3- and 8.7-fold increases of MNPCE frequency in the peripheral blood and bone marrow, respectively. Furthermore, when 3,6-DNBeP was intratracheally (i.t.) instilled to male ICR mice, 3,6-DNBeP at the highest dose of 0.1 mg/kg body exhibited 3.1-fold increase of DNA tail moment in the lungs at 3 h after the instillation compared to controls. The values of DNA tail moment at 9 and 24 h after the instillation were increased up to 3.5 and 4.2-fold, respectively. These data indicate that 3,6-DNBeP is genotoxic to mammalians in in vivo and suggest that 3,6-DNBeP may be a carcinogenic compound present in the human environment. © 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 588–594, 2013.

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