Quercetin attenuates lambda cyhalothrin-induced reproductive toxicity in male rats

Authors

  • Fatma Ben Abdallah,

    Corresponding author
    1. Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, University of sfax, Tunisia
    2. Laboratory of Molecular Human Genetics, Faculty of Medicine of Sfax, University of Sfax, Tunisia
    • Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, University of sfax, Tunisia. E-mail: fatma_isbs@yahoo.fr

    Search for more papers by this author
    • These authors contributed equally to this work.

  • Hamadi Fetoui,

    1. Animal Physiology Laboratory, Life Science Department, Sciences Faculty of Sfax, University of Sfax, Tunisia
    Search for more papers by this author
  • Nassira Zribi,

    1. Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, University of sfax, Tunisia
    2. Laboratory of Molecular Human Genetics, Faculty of Medicine of Sfax, University of Sfax, Tunisia
    Search for more papers by this author
  • Feiza Fakhfakh,

    1. Laboratory of Molecular Human Genetics, Faculty of Medicine of Sfax, University of Sfax, Tunisia
    Search for more papers by this author
  • Leila Keskes

    1. Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, University of sfax, Tunisia
    2. Laboratory of Molecular Human Genetics, Faculty of Medicine of Sfax, University of Sfax, Tunisia
    Search for more papers by this author

Abstract

The aim of this study was to evaluate the possible protective effects of Quercetin (Qe) against oxidative stress induced by λ cyhalothrin (LTC) in reproductive system. Thirty-two male rats were divided into four groups. First group was allocated as the control group. Second group was given a Qe alone while the third group received a LTC alone. Animals in the fourth group were given a Qe with LTC. Caudae epididymis was removed for sperm analysis. Lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and reduced glutathione (GSH) were determined in the testis. Additionally, the different histopathologic changes were observed in the testis of animals. LTC exposure significantly increased the abnormal morphology and LPO. On the contrary, sperm motility, viability and count, levels of GSH, and activities of SOD, CAT, GPx, and GST were significantly decreased compared to controls. Qe with LTC offset the decrease in functional sperm parameters, antioxidants enzymatic activities, and nonenzymatic antioxidant levels when compared with LTC-treated rats. Furthermore, LTC showed irregular seminiferous tubules containing only Sertoli cells and Qe with LTC caused regular seminiferous tubules showing spermatogenesis at level of spermatocytes. We conclude that LTC-induced oxidative stress and functional sperm parameters in male rats, and dietary of Qe attenuates the reproductive toxicity of LTC to restore the antioxidant system and sperm parameters in male rats. © 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 673–680, 2013.

Ancillary