Acute exposure to pure cylindrospermopsin results in oxidative stress and pathological alterations in tilapia (Oreochromis niloticus)
Version of Record online: 13 FEB 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 29, Issue 4, pages 371–385, April 2014
How to Cite
Puerto, M., Jos, A., Pichardo, S., Moyano, R., Blanco, A. and Cameán, A. M. (2014), Acute exposure to pure cylindrospermopsin results in oxidative stress and pathological alterations in tilapia (Oreochromis niloticus). Environ. Toxicol., 29: 371–385. doi: 10.1002/tox.21764
- Issue online: 6 MAR 2014
- Version of Record online: 13 FEB 2012
- Manuscript Accepted: 12 JAN 2012
- Manuscript Revised: 11 JAN 2012
- Manuscript Received: 19 AUG 2011
- Spanish Ministerio Ciencia e Innovación (CICYT). Grant Number: AGL2009-10026ALI
- Junta de Andalucía. Grant Number: P09-AGR-4672
- oxidative stress
Cylindrospermopsin (CYN) is increasingly recognized as a potential threat to drinking water safety, due to its ubiquity. This cyanotoxin has been found to cause toxic effects in mammals, and although fish could be in contact with this toxin, acute toxicity studies on fish are nonexistent. This is the first study showing that single doses of CYN pure standard (200 or 400 μg CYN/kg fish bw) by oral route (gavage) generate histopathological effects in fish (Tilapia—Oreochromis niloticus) exposed to the toxin under laboratory condition. Among the morphological changes, disorganized parenchymal architecture in the liver, dilated Bowman's space in the kidney, fibrolysis in the heart, necrotic enteritis in the intestines, and hemorrhages in the gills, were observed. Moreover, some oxidative stress biomarkers in the liver and kidney of tilapias were altered. Thus, CYN exposure induced increased protein oxidation products in both organs, NADPH oxidase activity was significantly increased with the kidney being the most affected organ, and decreased GSH contents were also detected in both organs, at the higher dose assayed. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 371–385, 2014.