Get access

Prenatal di-n-butyl phthalate exposure alters reproductive functions at adulthood in male rats

Authors

  • Nelli Giribabu,

    1. Department of Zoology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India, >
    Search for more papers by this author
  • Sri Bhashyam Sainath,

    1. Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
    Search for more papers by this author
  • Pamanji Sreenivasula Reddy

    Corresponding author
    1. Department of Zoology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India, >
    2. Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
    • Department of Zoology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India. E-mail: reddy_1955@yahoo.co.in

    Search for more papers by this author

Abstract

This study was aimed to investigate the reproductive health in adult male rats exposed to di-n-butyl phthalate (DBP) during embryonic development. Pregnant rats were injected with DBP and F1 male rats were weaned and on postnatal day 100, used for mating with normal cycling females to assess reproductive performance. After completion of cohabitation period, rats were analyzed for other reproductive end points. Transplacental exposure to DBP significantly decreased fertility in adult male rats. Prenatal exposure to DBP significantly decreased sperm density, number of motile sperms, viable sperms, and hypoosmotic swelling tail coiled sperms with an increase in morphological abnormalities in sperms. Testicular steroidogenic enzyme activity levels and serum testosterone levels were significantly decreased in rats exposed to DBP during embryonic development. In conclusion, transplacental exposure to DBP impairs male reproductive performance by decreasing steroidogenesis and spermatogenesis. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 534–544, 2014.

Ancillary