Role of propolis (bee glue) in improving histopathological changes of the kidney of rat treated with aluminum chloride

Authors

  • Ayman EL-meghawry EL-kenawy,

    1. Department of Molecular Biology, Genetic Engineering and Biotechnology Research Inst., Minufiya University, Egypt
    2. Department of pathology, College of medicine, Taif University, Kingdom of Saudi Arabia
    Search for more papers by this author
  • Hosam Eldin Hussein Osman,

    Corresponding author
    1. Department of pathology, College of medicine, Taif University, Kingdom of Saudi Arabia
    2. Department of Anatomy, Al Azhar University, Egypt
    Search for more papers by this author
  • Maha Hasan Daghestani

    1. Department of Zoology, King Saud University, Women Students-Medical Studies and Sciences Section, Kingdom of Saudi Arabia
    Search for more papers by this author

Abstract

Humans are frequently exposed to aluminum from various food additives, therapeutic treatments and the environment, and it can be potentially toxic. This study is aimed to elucidate the protective effects of propolis against aluminum chloride (AlCl3)-induced histopathological and immunohistochemical changes in kidney tissues of rats. Sixty Wistar Albino male rats (average weight 250–300 g) were divided into three equal groups. The first served as a negative control. The second received AlCl3 (34 mg/kg bw, 1/ 25 LD 50). The third were administered AlCl3 (34 mg/kg bw, 1/ 25 LD 50) plus propolis (50 mg/kg bw). Doses were given once daily via a gavage for 8 weeks every day. The results showed that shrunken glomeruli, intraglomerular congestion, loss of apical microvilli, degeneration of mitochondria and widened rough endoplasmic reticulum were also observed in the Proximal Convoluted Tubules of these animals. Treatment with propolis ameliorated the harmful effects of AlCl3; this was also proved histopathologically by the noticeable improvement in the renal tissues. There were also significant variations in the expressed of ki-67 and p53 proteins. It can be concluded that propolis may be promising as a natural therapeutic agent in AlCl3-induced renal toxicity and oxidative stress in rat kidneys. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 1000–1010, 2014.

Ancillary