Heavy metal influence on BDE-47 uptake in the human KERTr keratinocyte cell line

Authors

  • Yuan Kang,

    Corresponding author
    1. School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China
    2. Croucher Institute for Environmental Sciences and Department of Biology, Kowloon Tong, Hong Kong, People's Republic of China
    • School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China. E-mail: kangyuan407@gmail.com

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  • Yi Yin,

    1. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Guangzhou 510380, People's Republic of China
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  • Qiu Yun Zhang,

    1. School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China
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  • Lai Sheng Li,

    1. School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China
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  • Li Xuan Zeng,

    1. School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China
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  • Ji Wen Luo,

    1. School of Chemistry and Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, People's Republic of China
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  • Ming Hung Wong

    1. Croucher Institute for Environmental Sciences and Department of Biology, Kowloon Tong, Hong Kong, People's Republic of China
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Abstract

Significant correlations between concentrations of PBDEs and heavy metals were observed in the human body. However, there is a lack of evidence on the linkage between the uptake of heavy metals and PBDEs. This study is the first report on the BDE-47 uptake profile in a human cell line. Hg and As exposures to KERTr (human skin derived keratinocyte) did not significantly (p > 0.05) affect the uptake of BDE47, whereas Pb and Cd significantly (p < 0.05) affected the uptake of BDE-47 in KERTr. The change in Km was minor after exposure to all heavy metals. The maximum transport rate (Vmax) after exposure to Pb (Vmax: 5.23 ± 0.49) and As (Vmax: 4.95 ± 0.60) was significantly increased when compared with the background of the KERTr cell line (Vmax: 4.07 ± 0.35). Real-time RT-PCR indicated that OATP-B, OATP-D, and OATP-E were expressed in the KERTr cell line. The upregulation or downregulation of OATP B and D genes were minor after exposure to heavy metals, but the OATP E gene was upregulated by three to fourfold in KERTr cell line after exposure to Pb an Cd, which may explain the significant increase of uptake of BDE-47 in KERTr after exposures to Pb and Cd. This study indicated that the uptake effects should be considered when performing risk assessment of human exposure to PBDEs and heavy metal. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 354–361, 2014.

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