Effects of chlorpyrifos on the metabolome of the freshwater carp, Cyprinus Carpio
Article first published online: 30 AUG 2013
© 2013 Wiley Periodicals, Inc.
Volume 30, Issue 3, pages 253–260, March 2015
How to Cite
Kokushi, E., Uno, S., Pal, S. and Koyama, J. (2015), Effects of chlorpyrifos on the metabolome of the freshwater carp, Cyprinus Carpio. Environ. Toxicol., 30: 253–260. doi: 10.1002/tox.21903
- Issue published online: 28 JAN 2015
- Article first published online: 30 AUG 2013
- Manuscript Revised: 5 AUG 2013
- Manuscript Accepted: 5 AUG 2013
- Manuscript Received: 7 MAR 2013
- Environment Research and Technology Development fund of the Ministry of the Environment, Japan . Grant Number: RF-0909
- acetylcholinesterase inhibition
This study investigated the effects of waterborne chlorpyrifos with concentrations of 1 and 100 µg/L for L and H-groups, respectively, on metabolome profiles of carp plasma using 1H-NMR. Principal component analysis suggests that chlorpyrifos exposure firstly affected in L and H-groups on day 2 or 4, and followed a second effect in both exposure groups on day 14. Levels of metabolites related to the energy production in the body, such as glucose, glycerol, valine, leucine, isoleucine, lactate, alanine, 3-D-hydroxybutyrates and acetoacetate, significantly changed by exposures of chlorpyrifos. Those results suggest that energy production was severely affected in carp. The exposure could also be highly elevated ammonia levels especially in H-group due to severe convulsion in muscle caused by the inhibition of acetylcholinesterase activity. © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 253–260, 2015.