Responses of glutathione-related antioxidant defense system in serum of Nile tilapia (Oreochromis niloticus) exposed to sublethal concentration of methomyl and recovery pattern
Version of Record online: 25 NOV 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 30, Issue 4, pages 483–489, April 2015
How to Cite
Meng, S.-L., Qu, J.-H., Fan, L.-M., Qiu, L.-P., Chen, J.-Z. and Xu, P. (2015), Responses of glutathione-related antioxidant defense system in serum of Nile tilapia (Oreochromis niloticus) exposed to sublethal concentration of methomyl and recovery pattern. Environ. Toxicol., 30: 483–489. doi: 10.1002/tox.21925
- Issue online: 16 MAR 2015
- Version of Record online: 25 NOV 2013
- Manuscript Accepted: 10 NOV 2013
- Manuscript Received: 2 SEP 2013
- China Agriculture Research System. Grant Number: CARS-49
- Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences. Grant Number: 2013A0303
Tilapia were exposed to sublethal concentrations of 0, 0.2, 2, 20, or 200 μg/L for 30 days, and then transferred to methomyl-free water for 18 days. GST, GPx, GR, GSH, and GSSG in tilapia serum were examined at 0, 6, 12, 18, 24, and 30 days after methomyl exposure and at 18 days after transferring to methomyl-free water. There were no significant changes in antioxidants activities and contents in serum of tilapia exposed to 0.2 μg/L. Significant increases in GST, GR, GPx, and GSSG accompanied by a decrease in GSH were observed following methomyl exposure to 2, 20, or 200 μg/L, suggesting the presence of oxidative stress. Thus, it would appear the 0.2 μg/L methomyl might be considered the no observed adverse effect level. Recovery data showed that the effects produced by lower concentration of 20 μg/L were reversible but not at the higher 200 μg/L concentration. © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 483–489, 2015.