Time lapse microscopy observation of cellular structural changes and image analysis of drug treated cancer cells to characterize the cellular heterogeneity
Article first published online: 21 JAN 2014
© 2014 Wiley Periodicals, Inc.
Volume 30, Issue 6, pages 724–734, May/June 2015
How to Cite
Vaiyapuri, P. S., Ali, A. A., Mohammad, A. A., Kandhavelu, J. and Kandhavelu, M. (2015), Time lapse microscopy observation of cellular structural changes and image analysis of drug treated cancer cells to characterize the cellular heterogeneity. Environ. Toxicol., 30: 724–734. doi: 10.1002/tox.21950
- Issue published online: 17 MAY 2015
- Article first published online: 21 JAN 2014
- Manuscript Accepted: 8 JAN 2014
- Manuscript Revised: 4 JAN 2014
- Manuscript Received: 4 NOV 2013
- Bharathidasan University and Department of Science and Technology, India
- mammary carcinoma;
- complementary and alternative medicine;
- single cell imaging;
The effect of Calotropis gigantea latex (CGLX) on human mammary carcinoma cells is not well established. We present the results of this drug activity at total population and single cell level. CGLX inhibited the growth of MCF7 cancer cells at lower IC50 concentration (17 µL/mL). Microscopy of IC50 drug treated cells at 24 hr confirming the appearance of morphological characteristics of apoptotic and necrotic cells, associated with 70% of DNA damage. FACS analysis confirmed that, 10 and 20% of the disruption of cellular mitochondrial nature by at 24 and 48 h, respectively. Microscopic image analysis of total population level proved that MMP changes were statistically significant with P values. The cell to cell variation was confirmed by functional heterogeneity analysis which proves that CGLX was able to induce the apoptosis without the contribution of mitochondria. We conclude that CGLX inhibits cell proliferation, survival, and heterogeneity of pathways in human mammary carcinoma cells. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 724–734, 2015.