Effects of tetrabrombisphenol A on DNA integrity, oxidative stress, and sterlet (Acipenser ruthenus) spermatozoa quality variables
Article first published online: 24 JAN 2014
Copyright © 2014 Wiley Periodicals, Inc.
How to Cite
Linhartova, P., Gazo, I., Shaliutina-Kolesova, A., Hulak, M. and Kaspar, V. (2014), Effects of tetrabrombisphenol A on DNA integrity, oxidative stress, and sterlet (Acipenser ruthenus) spermatozoa quality variables. Environ. Toxicol.. doi: 10.1002/tox.21953
- Article first published online: 24 JAN 2014
- Manuscript Accepted: 8 JAN 2014
- Manuscript Revised: 2 JAN 2014
- Manuscript Received: 2 SEP 2013
- LO1205 (CENAKVA II) were obtained with a financial support from the MEYS of the CR under the NPU I program, CENAKVA CZ.1.05/2.1.00/01.0024 and GAJU 074/2013/Z
- DNA damage;
- comet assay;
- reactive oxygen species;
- spermatozoa motility
The sperm of sterlet (Acispenser ruthenus) was used to investigate the effect of the xenobiotic tetrabrombisphenol A (TBBPA) on sperm quality variables (ATP content, spermatozoa motility, and velocity), DNA integrity, and oxidative stress indices. Sperm was diluted to obtain a spermatozoa density of 5 × 108 cells/mL and exposed for 2 h to final concentrations of TBBPA (0.5, 1.75, 2.5, 5, and 10 μg/L). The oxidative stress indices, including lipid peroxidation, carbonyl derivatives of proteins, and antioxidant activity were significantly higher with increased concentrations of TBBPA. There was significantly less intracellular ATP in sperm samples at TBBPA concentrations of 2.5 μg/L and above. Spermatozoa velocity and percent motile sperm were significantly lower at each sampling time post-activation compared to controls. DNA damage expressed as percent DNA in Tail and Olive Tail moment was significantly higher with exposures ≥2.5 μg/L TBBPA. The results demonstrated that TBBPA and other xenobiotics can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system. This study confirmed that fish spermatozoa can be used in in vitro assays for monitoring residual pollution in aquatic environments. © 2014 Wiley Periodicals, Inc. Environ Toxicol, 2014.