Supported by: The National Natural Science Foundation of China (No. 81172623).
The role of Pten/Akt signaling pathway involved in BPA-induced apoptosis of rat sertoli cells
Article first published online: 25 JAN 2014
© 2014 Wiley Periodicals, Inc.
Volume 30, Issue 7, pages 793–802, July 2015
How to Cite
Wang, C., Fu, W., Quan, C., Yan, M., Liu, C., Qi, S. and Yang, K. (2015), The role of Pten/Akt signaling pathway involved in BPA-induced apoptosis of rat sertoli cells. Environ. Toxicol., 30: 793–802. doi: 10.1002/tox.21958
- Issue published online: 12 JUN 2015
- Article first published online: 25 JAN 2014
- Manuscript Accepted: 8 JAN 2014
- Manuscript Revised: 24 DEC 2013
- Manuscript Received: 17 JUL 2013
- The National Natural Science Foundation of China . Grant Number: 81172623
- apoptosis cells
Bisphenol-A (BPA), one of endocrine-disrupting chemicals, is a male reproductive toxicant. Previous studies have revealed the direct cytotoxicity of BPA in many cultured cells, such as mitotic aneuploidy in embryonic cells and somatic cells, and apoptosis in neurons and testicular Sertoli cells. To understand the action of BPA and assess its risk, the Pten/Akt pathway was investigated in cultured Sertoli cells to elucidate the mechanism of the reproductive effects of BPA. The results showed that over 50 μM BPA treatment could decrease the viability of Sertoli cells and cause more apoptosis. In addition, BPA could induce the increase in mRNA levels of Pten and Akt. The protein level of Pten was increased; however, the protein levels of phospho-Akt and procaspase-3 were decreased after BPA exposure. Taken together, observed results suggested that the Pten/Akt pathway might be involved in the apoptotic effects of BPA on Sertoli cells. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 793–802, 2015.