Profiling of rutin-mediated alleviation of cadmium-induced oxidative stress in Zygophyllum fabago
Version of Record online: 1 FEB 2014
© 2014 Wiley Periodicals, Inc.
Volume 30, Issue 7, pages 816–835, July 2015
How to Cite
Yildiztugay, E. and Ozfidan-Konakci, C. (2015), Profiling of rutin-mediated alleviation of cadmium-induced oxidative stress in Zygophyllum fabago. Environ. Toxicol., 30: 816–835. doi: 10.1002/tox.21960
- Issue online: 12 JUN 2015
- Version of Record online: 1 FEB 2014
- Manuscript Accepted: 19 JAN 2014
- Manuscript Revised: 16 JAN 2014
- Manuscript Received: 18 OCT 2013
- Selcuk University Scientific Research Projects Coordinating Office . Grant Number: 13401084.
- antioxidant system;
- cadmium stress;
- cadmium toxicity;
- oxidative stress;
- reactive oxygen species;
- Zygophyllum fabago
Zygophyllum fabago grows in arid, saline soil, or disturbed sites, such as former industrial or mining areas. This species is able to grow in coarse mineral substrates contaminated with heavy metals. To investigate the effects of the flavonoid rutin (Rtn) on certain heavy metal stress responses such as antioxidant defense systems and water status, seedlings were subjected to 100 and 200 μM CdCl2 treatment without or with 0.25 and 1 mM Rtn for 7 and 14 d (days). Cd stress decreased growth (RGR), water content (RWC), leaf osmotic potential (ΨΠ), and chlorophyll fluorescence, all of which could be partly alleviated by addition of Rtn. Activities of superoxide dismutase, peroxidase (POX), ascorbate peroxidase, and glutathione reductase increased within the first 7 d after exposure to Cd. However, failure of antioxidant defense in the scavenging of reactive oxygen species (ROS) was evidenced by an abnormal rise in superoxide anion radical ( ) and hydrogen peroxide contents and a decline in hydroxyl radical (OH•) scavenging activity, resulting in enhancement of lipid peroxidation (TBARS) as a marker of Cd-induced oxidative stress. However, exogenously applied Rtn considerably improved the stress tolerance of plants via a reduction in Cd accumulation, modulation of POX activity, increase of proline (Pro) content, decrease in TBARS and ROS content and consequent lowering of oxidative damage of membrane. Overall, 0.25 and 1 mM Rtn could protect Z. fabago from the harmful effects of 100 μM Cd-induced oxidative stress throughout the experiment. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 816–835, 2015.