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Crude extract of Rheum palmatum L inhibits migration and invasion of LS1034 human colon cancer cells acts through the inhibition of matrix metalloproteinase-2/-9 by MAPK signaling

Authors

  • Yi-Shih Ma,

    1. Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan, Republic of China
    2. Department of Chinese Medicine, Changhua Hospital, Changhua, Taiwan, Republic of China
    3. Department of Health, Executive Yuan, Changhua, Taiwan, Republic of China
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  • Yu-Ping Hsiao,

    1. Departments of Dermatology, Chung Shan Medical University Hospital, Taichung, Taiwan, Republic of China
    2. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, Republic of China
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  • Ju-Hwa Lin,

    1. Departments of Biological Science and Technology, China Medical University, Taichung, Taiwan, Republic of China
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  • Shu-Chun Hsu,

    1. Departments of Biological Science and Technology, China Medical University, Taichung, Taiwan, Republic of China
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  • Fu-Shin Chueh,

    1. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, Republic of China
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  • Shu-Wen Weng,

    1. Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan, Republic of China
    2. Department of Chinese Medicine, Taichung Hospital, Taichung, Taiwan, Republic of China
    3. Department of Health, Executive Yuan, Taichung, Taiwan, Republic of China
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  • Kuang-Chi Lai,

    1. School of Medicine, China Medical University, Taichung, Taiwan, Republic of China
    2. Department of Surgery, China Medical University Beigang Hospital, Yunlin, Taiwan, Republic of China
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  • Jaung-Geng Lin,

    Corresponding author
    1. Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan, Republic of China
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  • Jing-Gung Chung

    Corresponding author
    1. Departments of Biological Science and Technology, China Medical University, Taichung, Taiwan, Republic of China
    2. Department of Biotechnology, Asia University, Taichung, Taiwan, Republic of China
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ABSTRACT

Crude extract of Rheum palmatum L. (CERP) has been used to treat different diseases in the Chinese population for decades. In this study, we investigated the anti-metastasis effects of CERP on LS1034 human colorectal cancer cells in vitro and examined potential mechanisms of its effects. CERP significantly inhibited cell migration and invasion of LS1034 cells. We also found that CERP inhibited protein levels of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9), and cytosolic NF-kB p65, RHO A, ROCK 1. Furthermore, we found CERP inhibited protein levels of GRB2, SOS1, MKK7, FAK, Rho A, ROCK 1, VEGF, PKC, AKT, phosphor-AKT (Thr308), Cyclin D, iNOS, COX2, NF-kB p65, p-ERK1/2, p-JNK1/2, p-p38, p-c-jun, MMP-2, MMP-9, MMP-1, MMP-7, MMP-10, UPA and increased the protein level of Ras in LS1034 cells. In conclusion, our results suggest that CERP may be used as a novel anti-metastasis agent for the treatment of human colon cancer cells. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 852–863, 2015.

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