These authors contributed equally to this work.
Hyparrhenia hirta: A potential protective agent against hematotoxicity and genotoxicity of sodium nitrate in adult rats
Version of Record online: 17 APR 2014
© 2014 Wiley Periodicals, Inc.
Volume 30, Issue 11, pages 1275–1284, November 2015
How to Cite
Bouaziz-Ketata, H., Salah, G. B., Mahjoubi, A., Aidi, Z., Kallel, C., Kammoun, H., Fakhfakh, F. and Zeghal, N. (2015), Hyparrhenia hirta: A potential protective agent against hematotoxicity and genotoxicity of sodium nitrate in adult rats. Environ. Toxicol., 30: 1275–1284. doi: 10.1002/tox.21998
- Issue online: 13 OCT 2015
- Version of Record online: 17 APR 2014
- Manuscript Accepted: 4 APR 2014
- Manuscript Revised: 31 MAR 2014
- Manuscript Received: 17 AUG 2013
- sodium nitrate;
- Hyparrhenia hirta;
- antioxidant status
The present study was carried out to examine the adverse hematotoxic and genotoxic effects of water nitrate pollution on male adult rats and the use of hyparrhenia hirta methanolic extract in alleviating these effects. Sodium nitrate (NaNO3) was administered to adult rats by oral gavage at a dose of 400 mg kg−1 bw daily for 50 days, while hyparrhenia hirta methanolic extract was given by drinking water at a dose of 1.5 mg mL−1 (200 mg kg−1 bw). The NaNO3-treated group showed a significant decrease in red blood cell count, hemoglobin and hematocrit and a significant increase in total white blood cell, in neutrophil and eosinophil counts. Platelet count, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration remained unchanged in treated groups compared to those of controls. Meanwhile, the results showed a marked reduction in the antioxidant enzyme activities, such as superoxide dismutase, catalase, and glutathione peroxidase, along with an elevation in the level of lipid peroxidation and a reduction in the total glutathione content, indicating the induction of oxidative stress in the erythrocytes of NaNO3-treated group. Interestingly, NaNO3 treatment showed a significant increase in the frequencies of total chromosomal aberrations, aberrant metaphases and micronucleus in bone-marrow cells. The oxidative stress induced by nitrate treatment might be the major cause for chromosomal rearrangements as free radicals leading to DNA damage. Hyparrhenia hirta methanolic extract appeared to be effective against hematotoxic and genotoxic changes induced by nitrate, as evidenced by the improvement of the markers cited above. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1275–1284, 2015.