Supported by: China Medical University, Taichung, Taiwan (No. CMU100-ASIA-04). Experiments and data analysis were performed in part through the use of the Medical Research Core Facilities Center, Office of Research & Development at China medical University, Taichung, Taiwan, R.O.C.
Alpha-phellandrene-induced DNA damage and affect DNA repair protein expression in WEHI-3 murine leukemia cells in vitro
Version of Record online: 23 MAY 2014
© 2014 Wiley Periodicals, Inc.
Volume 30, Issue 11, pages 1322–1330, November 2015
How to Cite
Lin, J.-J., Wu, C.-C., Hsu, S.-C., Weng, S.-W., Ma, Y.-S., Huang, Y.-P., Lin, J.-G. and Chung, J.-G. (2015), Alpha-phellandrene-induced DNA damage and affect DNA repair protein expression in WEHI-3 murine leukemia cells in vitro. Environ. Toxicol., 30: 1322–1330. doi: 10.1002/tox.22003
- Issue online: 13 OCT 2015
- Version of Record online: 23 MAY 2014
- Manuscript Accepted: 9 MAY 2014
- Manuscript Revised: 28 APR 2014
- Manuscript Received: 10 MAR 2014
- WEHI-3 leukemia cells;
- DNA damage and repair
Although there are few reports regarding α-phellandrene (α-PA), a natural compound from Schinus molle L. essential oil, there is no report to show that α-PA induced DNA damage and affected DNA repair associated protein expression. Herein, we investigated the effects of α-PA on DNA damage and repair associated protein expression in murine leukemia cells. Flow cytometric assay was used to measure the effects of α-PA on total cell viability and the results indicated that α-PA induced cell death. Comet assay and 4,6-diamidino-2-phenylindole dihydrochloride staining were used for measuring DNA damage and condensation, respectively, and the results indicated that α-PA induced DNA damage and condensation in a concentration-dependent manner. DNA gel electrophoresis was used to examine the DNA damage and the results showed that α-PA induced DNA damage in WEHI-3 cells. Western blotting assay was used to measure the changes of DNA damage and repair associated protein expression and the results indicated that α-PA increased p-p53, p-H2A.X, 14-3-3-σ, and MDC1 protein expression but inhibited the protein of p53, MGMT, DNA-PK, and BRCA-1. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1322–1330, 2015.