These authors contributed equally to this work.
Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo
Article first published online: 28 MAY 2014
© 2014 Wiley Periodicals, Inc.
Volume 30, Issue 11, pages 1343–1353, November 2015
How to Cite
Hung, F.-M., Shang, H.-S., Tang, N.-Y., Lin, J.-J., Lu, K.-W., Lin, J.-P., Ko, Y.-C., Yu, C.-C., Wang, H.-L., Liao, J.-C., Lu, H.-F. and Chung, J.-G. (2015), Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo. Environ. Toxicol., 30: 1343–1353. doi: 10.1002/tox.22005
- Issue published online: 13 OCT 2015
- Article first published online: 28 MAY 2014
- Manuscript Accepted: 11 MAY 2014
- Manuscript Received: 27 JAN 2014
- Taiwan Department of Health, China Medical University Hospital Cancer Research Center of Excellence . Grant Number: DOH101-TD-C-111-005
- China Medical University, Taichung, Taiwan . Grant Number: CMU97-127
- WEHI-3 leukemia cells;
- immune responses;
- leukemic mice
Diallyl trisulfide (DATS), a chemopreventive dietary constituent and extracted from garlic, has been shown to against cultured many types of human cancer cell liens but the fate of apoptosis in murine leukemia cells in vitro and immune responses in leukemic mice remain elusive. Herein, we clarified the actions of DATS on growth inhibition of murine leukemia WEHI-3 cells in vitro and used WEHI-3 cells to generate leukemic mice in vivo, following to investigate the effects of DATS in animal model. In in vitro study, DATS induced apoptosis of WEHI-3 cells through the G0/G1 phase arrest and induction of caspase-3 activation. In in vivo study DATS decreased the weight of spleen of leukemia mice but did not affect the spleen weight of normal mice. DATS promoted the immune responses such as promotions of the macrophage phagocytosis and NK cell activities in WEHI-3 leukemic and normal mice. However, DATS only promotes NK cell activities in normal mice. DATS increases the surface markers of CD11b and Mac-3 in leukemia mice but only promoted CD3 in normal mice. In conclusion, the present study indicates that DATS induces cell death through induction of apoptosis in mice leukemia WHEI-3 cells. DATS also promotes immune responses in leukemia and normal mice in vivo. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1343–1353, 2015.