Rutin improves endotoxin-induced acute lung injury via inhibition of iNOS and VCAM-1 expression

Authors

  • Yi-Chun Huang,

    1. School of Health, National Taichung University of Science and Technology, Taichung, Taiwan
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    • *These authors contributed equally to this work.

  • Chi-Ting Horng,

    1. Medical Education Center, Kaohsiung Armed Forced General Hospital, Kaohsiung, Taiwan
    2. Department of Ophthalmology, Kaohsiung Armed Forced General Hospital, Kaohsiung, Taiwan
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  • Shyan-Tarng Chen,

    1. School of Optometry, Chung Shan Medical University, Taichung, Taiwan
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  • Shiuan-Shinn Lee,

    1. School of Public Health, Chung Shan Medical University, Taichung, Taiwan
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    • *These authors contributed equally to this work.

  • Ming-Ling Yang,

    1. Department of Anatomy, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
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  • Chien-Ying Lee,

    1. Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
    2. Department of Pharmacy, Chung Shan Medical University Hospital, Taichung, Taiwan
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  • Wu-Hsien Kuo,

    1. Department of Medicine, Kaohsiung Armed Forced General Hospital,Taiwan, Kaohsiung, Taiwan
    2. Department of Medicine, Tri-Service General Hospital, Taipei, Taiwan
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  • Chung-Hsin Yeh,

    1. Department of Neurology, Show Chwan Memorial Hospital, Changhua, Taiwan
    2. Department of Nursing, College of Medicine & Nursing, Hung Kuang University, Taichung, Taiwan
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  • Yu-Hsiang Kuan

    Corresponding author
    1. Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
    2. Department of Pharmacy, Chung Shan Medical University Hospital, Taichung, Taiwan
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ABSTRACT

Endotoxins exist anywhere including in water pools, dust, humidifier systems, and machining fluids. The major causal factor is endotoxins in many serious diseases, such as fever, sepsis, multi-organ failure, meningococcemia, and severe morbidities like neurologic disability, or hearing loss. Endotoxins are also called lipopolysaccharide (LPS) and are important pathogens of acute lung injury (ALI). Rutin has potential beneficial effects including anti-inflammation, antioxidation, anti-hyperlipidemia, and anti-platelet aggregation. Pre-treatment with rutin inhibited LPS-induced neutrophil infiltration in the lungs. LPS-induced expression of vascular cell adhesion molecule (VCAM)-1 and inducible nitric oxide synthase (iNOS) was suppressed by rutin, but there was no influence on expression of intercellular adhesion molecule-1 and cyclooxygenase-2. In addition, activation of the nuclear factor (NF)κB was reduced by rutin. Furthermore, we found that the inhibitory concentration of rutin on expression of VCAM-1 and iNOS was similar to NFκB activation. In conclusion, rutin is a potential protective agent for ALI via inhibition of neutrophil infiltration, expression of VCAM-1 and iNOS, and NFκB activation. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 185–191, 2016.

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