Fetal fraction in maternal plasma cell-free DNA at 11–13 weeks' gestation: relation to maternal and fetal characteristics
Article first published online: 4 DEC 2012
Copyright © 2012 ISUOG. Published by John Wiley & Sons, Ltd.
Ultrasound in Obstetrics & Gynecology
Volume 41, Issue 1, pages 26–32, January 2013
How to Cite
Ashoor, G., Syngelaki, A., Poon, L. C. Y., Rezende, J. C. and Nicolaides, K. H. (2013), Fetal fraction in maternal plasma cell-free DNA at 11–13 weeks' gestation: relation to maternal and fetal characteristics. Ultrasound Obstet Gynecol, 41: 26–32. doi: 10.1002/uog.12331
- Issue published online: 25 DEC 2012
- Article first published online: 4 DEC 2012
- Accepted manuscript online: 25 OCT 2012 10:23AM EST
- Manuscript Accepted: 18 OCT 2012
- cell-free DNA;
- fetal fraction;
- first-trimester screening;
- non-invasive prenatal testing;
- trisomy 21
To examine the possible effects of maternal and fetal characteristics on the fetal fraction in maternal plasma cell-free (cf) DNA at 11–13 weeks' gestation and estimate the proportion of pregnancies at high risk of non-invasive prenatal testing (NIPT) failure because the fetal fraction is less than 4%.
In 1949 singleton pregnancies at 11–13 weeks' gestation cf-DNA was extracted from maternal plasma. Chromosome-selective sequencing of non-polymorphic and polymorphic loci, where fetal alleles differ from maternal alleles, was used to determine the proportion of cf-DNA that was of fetal origin. Multivariable regression analysis was used to determine significant predictors of the fetal fraction among maternal and fetal characteristics.
The fetal fraction decreased with increased maternal weight, it was lower in women of Afro-Caribbean origin than in Caucasians and increased with fetal crown–rump length, serum pregnancy-associated plasma protein-A, serum free β-human chorionic gonadotropin, smoking and trisomy 21 karyotype. The median fetal fraction was 10.0% (interquartile range, 7.8–13.0%) and this decreased with maternal weight from 11.7% at 60 kg to 3.9% at 160 kg. The estimated proportion with fetal fraction below 4% increased with maternal weight from 0.7% at 60 kg to 7.1% at 100 kg and 51.1% at 160 kg.
Fetal fraction in maternal plasma cf-DNA is affected by maternal and fetal characteristics.Copyright © 2012 ISUOG. Published by John Wiley & Sons, Ltd.