Small molecule screening in zebrafish: swimming in potential drug therapies
Article first published online: 28 FEB 2012
Copyright © 2012 Wiley Periodicals, Inc.
Wiley Interdisciplinary Reviews: Developmental Biology
Volume 1, Issue 3, pages 459–468, May/June 2012
How to Cite
Tamplin, O. J., White, R. M., Jing, L., Kaufman, C. K., Lacadie, S. A., Li, P., Taylor, A. M. and Zon, L. I. (2012), Small molecule screening in zebrafish: swimming in potential drug therapies. WIREs Dev Biol, 1: 459–468. doi: 10.1002/wdev.37
- Issue published online: 17 APR 2012
- Article first published online: 28 FEB 2012
Phenotype-driven chemical genetic screens in zebrafish have become a proven approach for both dissection of developmental mechanisms and discovery of potential therapeutics. A library of small molecules can be arrayed into multiwell plates containing zebrafish embryos. The embryo becomes a whole organism in vivo bioassay that can produce a phenotype upon treatment. Screens have been performed that are based simply on the morphology of the embryo. Other screens have scored complex phenotypes using whole mount in situ hybridization, fluorescent transgenic reporters, and even tracking of embryo movement. The availability of many well-characterized zebrafish mutants has also enabled the discovery of chemical suppressors of genetic phenotypes. Importantly, the application of chemical libraries that already contain FDA-approved drugs has allowed the rapid translation of hits from zebrafish chemical screens to clinical trials. WIREs Dev Biol 2012, 1:459–468. doi: 10.1002/wdev.37
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