Engineered viral nanoparticles for flow cytometry and fluorescence microscopy applications
Article first published online: 14 JUN 2012
Copyright © 2012 Wiley Periodicals, Inc.
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
Volume 4, Issue 5, pages 511–524, September/October 2012
How to Cite
Robertson, K. L. and Liu, J. L. (2012), Engineered viral nanoparticles for flow cytometry and fluorescence microscopy applications. WIREs Nanomed Nanobiotechnol, 4: 511–524. doi: 10.1002/wnan.1177
- Issue published online: 17 AUG 2012
- Article first published online: 14 JUN 2012
Viral nanoparticles (VNPs) are attractive platforms for use in the biotechnology and biomedical fields because of their biological nature. A wide variety of these particles, labeled with fluorescent reporters, have been characterized using flow cytometry and cellular imaging techniques. Fluorescence microscopy allows the direct observation of VNPs on the cell surface or inside the membrane as well as the cellular localization of the nanoparticles while flow cytometry allows the statistical quantification of nanoparticle uptake and targeting specificity. These techniques are essential when characterizing the properties of VNPs and provide information toward the use of VNPs for targeting, imaging, and/or cargo delivery. WIREs Nanomed Nanobiotechnol 2012, 4:511–524. doi: 10.1002/wnan.1177
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This article is a U.S. Government work, and as such, is in the public domain in the United States of America.