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Microfabricated platforms to modulate and monitor T cell synapse assembly

Authors

  • Hong-Ryul Jung,

    1. School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
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  • Jong-Cheol Choi,

    1. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
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  • Wonhwa Cho,

    1. World Class University Program, Division of Integrative Biosciences and Biotechnology (IBB), Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
    2. Department of Chemistry (M/C 111), University of Illinois at Chicago, Chicago, IL, USA
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  • Junsang Doh

    Corresponding author
    1. School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
    2. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
    3. World Class University Program, Division of Integrative Biosciences and Biotechnology (IBB), Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
    • School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea
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Abstract

Immunological synapse (IS) is a complex supramolecular structure formed at the interface between T cells and antigen presenting cells (APCs) during T cell antigen recognition. Microfabricated platforms have made great contributions to our understanding of the assembly dynamics and functional roles of the T cell synapses over the last decade. Here, we review three different types of microfabricated platforms developed to modulate and monitor the T cell synapse assembly. Firstly, multi-protein micropatterned surfaces presenting key ligands for T cell activation that can modulate the spatial distribution of receptors/signaling molecules in T cell synapses are described. Secondly, micropatterned supported bilayers that can modulate the dynamics of T cell receptor (TCR) microclusters are introduced. Lastly, T-APC pair arrays that allow for improved fluorescence live cell imaging are discussed. WIREs Nanomed Nanobiotechnol 2013, 5:67–74. doi: 10.1002/wnan.1182

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