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Protein nanomachines assembly modes: cell-free expression and biochip perspectives
Version of Record online: 24 JUL 2013
© 2013 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals, Inc.
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Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
Volume 5, Issue 6, pages 613–628, November/December 2013
How to Cite
Daube, S. S. and Bar-Ziv, R. H. (2013), Protein nanomachines assembly modes: cell-free expression and biochip perspectives. WIREs Nanomed Nanobiotechnol, 5: 613–628. doi: 10.1002/wnan.1234
- Issue online: 15 OCT 2013
- Version of Record online: 24 JUL 2013
- Manuscript Accepted: 26 JUN 2013
- Manuscript Revised: 24 JUN 2013
- Manuscript Received: 6 MAR 2013
- Israel Science Foundation
- BSF-US-Israel Binational Science Foundation
- Minerva Foundation
- Volkswagen Foundation
Large macromolecular assemblies are widespread in all cell types with diverse structural and functional roles. Whether localized to membranes, nuclei, or cytoplasm, multimeric protein–nucleic acid complexes may be viewed as sophisticated nanomachines, an inspiration to chemical design. The formation of large biological assemblies follows a complex and hierarchical self-assembly process via ordered molecular recognition events. Serving a paradigm for biological assembly, extensive past studies of T4 bacteriophage and bacterial ribosomes by many groups have been revealing distinct design strategies, yet these two very different multimeric complexes share common mechanistic motifs. An emerging biochip approach highlights two conceptual notions to promote the study of assembly pathways: cell-free expression provides coupling between synthesis and assembly; surface anchoring allows high-resolution imaging of structural intermediates and opens up opportunities for rewiring a network by defining unnatural scaffolds for synthetic design applications. WIREs Nanomed Nanobiotechnol 2013, 5:613–628. doi: 10.1002/wnan.1227
Conflict of interest: The authors have declared no conflicts of interest for this article.
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