Plasmonic antennas and zero-mode waveguides to enhance single molecule fluorescence detection and fluorescence correlation spectroscopy toward physiological concentrations
Article first published online: 24 FEB 2014
© 2014 Wiley Periodicals, Inc.
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
Volume 6, Issue 3, pages 268–282, May/June 2014
How to Cite
Punj, D., Ghenuche, P., Moparthi, S. B., de Torres, J., Grigoriev, V., Rigneault, H. and Wenger, J. (2014), Plasmonic antennas and zero-mode waveguides to enhance single molecule fluorescence detection and fluorescence correlation spectroscopy toward physiological concentrations. WIREs Nanomed Nanobiotechnol, 6: 268–282. doi: 10.1002/wnan.1261
- Issue published online: 11 APR 2014
- Article first published online: 24 FEB 2014
- European Research Council under the European Union's Seventh Framework Programme. Grant Number: FP7/2007-2013
- ExtendFRET. Grant Number: ERC Grant agreement 278242
- NanoVista. Grant Number: 288263
Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as Förster fluorescence resonance energy transfer (FRET) or fluorescence correlation spectroscopy (FCS) are limited by diffraction to the nanomolar concentration range, far below the physiological micromolar concentration range where most biological reaction occur. To breach the diffraction limit, zero-mode waveguides (ZMW) and plasmonic antennas exploit the surface plasmon resonances to confine and enhance light down to the nanometer scale. The ability of plasmonics to achieve extreme light concentration unlocks an enormous potential to enhance fluorescence detection, FRET, and FCS. Single molecule spectroscopy techniques greatly benefit from ZMW and plasmonic antennas to enter a new dimension of molecular concentration reaching physiological conditions. The application of nano-optics to biological problems with FRET and FCS is an emerging and exciting field, and is promising to reveal new insights on biological functions and dynamics.
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Conflict of interest: The authors have declared no conflicts of interest for this article.