This article is a U.S. Government work, and as such, is in the public domain in the Unites States of America.
Probing dynamic fluorescence properties of single and clustered quantum dots toward quantitative biomedical imaging of cells †
Article first published online: 28 OCT 2009
Copyright © 2009 John Wiley & Sons, Inc.
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
Volume 2, Issue 1, pages 48–58, January/February 2010
How to Cite
Kang, H.-G., Tokumasu, F., Clarke, M., Zhou, Z., Tang, J., Nguyen, T. and Hwang, J. (2010), Probing dynamic fluorescence properties of single and clustered quantum dots toward quantitative biomedical imaging of cells . WIREs Nanomed Nanobiotechnol, 2: 48–58. doi: 10.1002/wnan.62
- Issue published online: 14 DEC 2009
- Article first published online: 28 OCT 2009
We present results on the dynamic fluorescence properties of bioconjugated nanocrystals or quantum dots (QDs) in different chemical and physical environments. A variety of QD samples was prepared and compared: isolated individual QDs, QD aggregates, and QDs conjugated to other nanoscale materials, such as single-wall carbon nanotubes (SWCNTs) and human erythrocyte plasma membrane proteins. We discuss plausible scenarios to explain the results obtained for the fluorescence characteristics of QDs in these samples, especially for the excitation time-dependent fluorescence emission from clustered QDs. We also qualitatively demonstrate enhanced fluorescence emission signals from clustered QDs and deduce that the band 3 membrane proteins in erythrocytes are clustered. This approach is promising for the development of QD-based quantitative molecular imaging techniques for biomedical studies involving biomolecule clustering. WIREs Nanomed Nanobiotechnol 2010 2 48–58
For further resources related to this article, please visit the WIREs website.