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Probing dynamic fluorescence properties of single and clustered quantum dots toward quantitative biomedical imaging of cells †

  1. Hyeong-Gon Kang1,
  2. Fuyuki Tokumasu2,
  3. Matthew Clarke1,
  4. Zhenping Zhou1,
  5. Jianyong Tang1,
  6. Tinh Nguyen1,
  7. Jeeseong Hwang1,*

Article first published online: 28 OCT 2009

DOI: 10.1002/wnan.62

Issue

Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology

Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology

Volume 2, Issue 1, pages 48–58, January/February 2010

Additional Information

How to Cite

Kang, H.-G., Tokumasu, F., Clarke, M., Zhou, Z., Tang, J., Nguyen, T. and Hwang, J. (2010), Probing dynamic fluorescence properties of single and clustered quantum dots toward quantitative biomedical imaging of cells . WIREs Nanomed Nanobiotechnol, 2: 48–58. doi: 10.1002/wnan.62

Author Information

  1. 1

    National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA

  2. 2

    National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway, Bethesda, MD 20892, USA

*National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA

  1. †

    This article is a U.S. Government work, and as such, is in the public domain in the Unites States of America.

Publication History

  1. Issue published online: 14 DEC 2009
  2. Article first published online: 28 OCT 2009

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Abstract

We present results on the dynamic fluorescence properties of bioconjugated nanocrystals or quantum dots (QDs) in different chemical and physical environments. A variety of QD samples was prepared and compared: isolated individual QDs, QD aggregates, and QDs conjugated to other nanoscale materials, such as single-wall carbon nanotubes (SWCNTs) and human erythrocyte plasma membrane proteins. We discuss plausible scenarios to explain the results obtained for the fluorescence characteristics of QDs in these samples, especially for the excitation time-dependent fluorescence emission from clustered QDs. We also qualitatively demonstrate enhanced fluorescence emission signals from clustered QDs and deduce that the band 3 membrane proteins in erythrocytes are clustered. This approach is promising for the development of QD-based quantitative molecular imaging techniques for biomedical studies involving biomolecule clustering. WIREs Nanomed Nanobiotechnol 2010 2 48–58

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