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Cloning and characterization of a dextranase gene from Lipomyces starkeyi and its expression in Saccharomyces cerevisiae

  1. Hee-Kyoung Kang1,2,
  2. Seung Heuk Kim3,
  3. Ji-Young Park1,4,
  4. Xing-Ji Jin1,5,
  5. Deok-Kun Oh6,
  6. Seong Soo Kang7,
  7. Doman Kim1,8,9,*

Article first published online: 9 NOV 2005

DOI: 10.1002/yea.1311

Issue

Yeast

Yeast

Volume 22, Issue 15, pages 1239–1248, November 2005

Additional Information

How to Cite

Kang, H.-K., Kim, S. H., Park, J.-Y., Jin, X.-J., Oh, D.-K., Soo Kang, S. and Kim, D. (2005), Cloning and characterization of a dextranase gene from Lipomyces starkeyi and its expression in Saccharomyces cerevisiae. Yeast, 22: 1239–1248. doi: 10.1002/yea.1311

Author Information

  1. 1

    Laboratory of Functional Carbohydrate Enzymes and Microbial Genomics, Institute of Bioindustrial Technology, Chonnam National University, Gwang-Ju 500-757, South Korea

  2. 2

    Engineering Research Institute, Chonnam National University, Gwang-Ju 500-757, South Korea

  3. 3

    Lifenza Co. Ltd, Gyeonggi-Do 431-062, South Korea

  4. 4

    Department of Materials and Biochemical Engineering, Chonnam National University, Gwang-Ju 500-757, South Korea

  5. 5

    Department of Fine Chemical Engineering, Chonnam National University, Gwang-Ju 500-757, South Korea

  6. 6

    Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, South Korea

  7. 7

    College of Veterinary Medicine, Chonnam National University, Gwang-Ju 500-757, South Korea

  8. 8

    School of Biological Sciences and Technology, and Research Institute for Catalysis, Chonnam National University, Gwang-Ju 500-757, South Korea

  9. 9

    Biology Research Center for Industrial Accelerator, Dongshin University, Naju, Jeollanamdo 520-714, South Korea

*School of Biological Sciences and Technology, Chonnam National University, Gwang-Ju 500-757, South Korea.

Publication History

  1. Issue published online: 9 NOV 2005
  2. Article first published online: 9 NOV 2005
  3. Manuscript Accepted: 12 SEP 2005
  4. Manuscript Received: 29 MAR 2005

Funded by

  • Ministry of Science and Technology, South Korea. Grant Number: MG05-0301-1-0

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Keywords:

  • dextranase;
  • Lipomyces starkeyi;
  • Saccharomyces cerevisiae;
  • expression

Abstract

A dextranase-encoding cDNA from L. starkeyi KSM22 was isolated and characterized. The 2052 bp cDNA fragment (lsd1) harbouring the dextranase gene exhibited one open reading frame (ORF) composed of 1824 bp flanked by a 41 bp 5′-UTR and a 184 bp 3′-UTR, including a 27 bp poly(A) tail. The lsd1 gene contains no introns. The open reading frame encodes a 608 amino acid polypeptide (LSD1) with a 67.6 kDa predicted molecular mass. There was a 77% deduced amino acid sequence identity between the LSD1 dextranase and the dextranase from Penicillium minioluteum. The primary structure of LSD1 dextranase exhibits distant similarity with the enzymes of the glycosyl hydrolase family 49 that comprises Penicillium dextranase. The optimum pH of LSD1 was 6.0 and the optimum temperature was 37 °C. LSD1 dextranase activity was substantially abolished by exposure to 1 mM Hg2+, Ag3+ and Mn2+. LSD1 exhibited high hydrolysing activity towards dextran (100%), soluble starch (22%) and mutan (8%). Copyright © 2005 John Wiley & Sons, Ltd.

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