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The GPI-anchored Gas and Crh families are fungal antigens

  1. Javier Arroyo1,
  2. Jaqueline Sarfati2,
  3. Marie Thérése Baixench3,
  4. Enrico Ragni4,
  5. Marivi Guillén1,
  6. José Manuel Rodriguez-Peña1,
  7. Laura Popolo4,
  8. Jean Paul Latgé2,*

Article first published online: 2 APR 2007

DOI: 10.1002/yea.1480

Issue

Yeast

Yeast

Special Issue: 3rd International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis, August 31-September 5, 2006, Heidelberg, Germany

Volume 24, Issue 4, pages 289–296, April 2007

Additional Information

How to Cite

Arroyo, J., Sarfati, J., Baixench, M. T., Ragni, E., Guillén, M., Rodriguez-Peña, J. M., Popolo, L. and Latgé, J. P. (2007), The GPI-anchored Gas and Crh families are fungal antigens. Yeast, 24: 289–296. doi: 10.1002/yea.1480

Author Information

  1. 1

    Departamento Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain

  2. 2

    Unité des Aspergillus, Institut Pasteur, 75015 Paris, France

  3. 3

    Laboratoire de Parasitologie-Mycologie, Hôpital Cochin, 75014 Paris, France

  4. 4

    Università degli Studi di Milano, Dipartimento di Scienze Biomolecolari e Biotecnologie, 20133 Milano, Italy

*Unité des Aspergillus, Institut Pasteur, 25 Rue du Dr Roux, 75015 Paris, France.

Publication History

  1. Issue published online: 2 APR 2007
  2. Article first published online: 2 APR 2007
  3. Manuscript Accepted: 30 JAN 2007
  4. Manuscript Received: 28 NOV 2006

Funded by

  • European Union. Grant Numbers: MRTN-CT-2004-512481, PRIN 2005, LSBH-CT-2004-511952, CICYT BIO2004-06376, LSHB-CT-2004-511952

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Keywords:

  • glycosyltransferase;
  • aspergillus;
  • candida;
  • diagnosis;
  • GPI

Abstract

The cell wall is the first interface between a fungus and its extracellular environment. Glycosyltransferases involved in the formation and dynamic remodelling of the polysaccharide network of the cell wall have recently been identified. The best characterized ones belong to the Gas family, which elongates β(1,3)-glucans, and to the Crh family, which are involved in the cross-linking of chitin to β(1,6)-glucan. All these proteins carry a glycosylphosphatidylinositol (GPI) anchor. In this work, we show that recombinant soluble forms of Gas1–5 and Crh1p from Saccharomyces cerevisiae and their orthologous proteins Gel1-Gel2 and Crf1 from Aspergillus fumigatus are specifically recognized by antibodies present in the sera of patients with Aspergillus or Candida infections. Quantification of the antibody titres against recombinant Gas/Gel and Crh/Crf proteins separated aspergilloma and candidiasis patients from non-infected individuals. Cross-reactivity was seen between the antibody response of patients with aspergillosis and candidiasis towards the Gas/Gel and Crh/Crf proteins. These results suggest that GPI-anchored cross-linking enzymes are relevant immunologically reactive constituents of the cell wall that may play a role during human fungal infections. Copyright © 2007 John Wiley & Sons, Ltd.

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