The GPI-anchored Gas and Crh families are fungal antigens
Article first published online: 2 APR 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Special Issue: 3rd International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis, August 31-September 5, 2006, Heidelberg, Germany
Volume 24, Issue 4, pages 289–296, April 2007
How to Cite
Arroyo, J., Sarfati, J., Baixench, M. T., Ragni, E., Guillén, M., Rodriguez-Peña, J. M., Popolo, L. and Latgé, J. P. (2007), The GPI-anchored Gas and Crh families are fungal antigens. Yeast, 24: 289–296. doi: 10.1002/yea.1480
- Issue published online: 2 APR 2007
- Article first published online: 2 APR 2007
- Manuscript Accepted: 30 JAN 2007
- Manuscript Received: 28 NOV 2006
- European Union. Grant Numbers: MRTN-CT-2004-512481, PRIN 2005, LSBH-CT-2004-511952, CICYT BIO2004-06376, LSHB-CT-2004-511952
The cell wall is the first interface between a fungus and its extracellular environment. Glycosyltransferases involved in the formation and dynamic remodelling of the polysaccharide network of the cell wall have recently been identified. The best characterized ones belong to the Gas family, which elongates β(1,3)-glucans, and to the Crh family, which are involved in the cross-linking of chitin to β(1,6)-glucan. All these proteins carry a glycosylphosphatidylinositol (GPI) anchor. In this work, we show that recombinant soluble forms of Gas1–5 and Crh1p from Saccharomyces cerevisiae and their orthologous proteins Gel1-Gel2 and Crf1 from Aspergillus fumigatus are specifically recognized by antibodies present in the sera of patients with Aspergillus or Candida infections. Quantification of the antibody titres against recombinant Gas/Gel and Crh/Crf proteins separated aspergilloma and candidiasis patients from non-infected individuals. Cross-reactivity was seen between the antibody response of patients with aspergillosis and candidiasis towards the Gas/Gel and Crh/Crf proteins. These results suggest that GPI-anchored cross-linking enzymes are relevant immunologically reactive constituents of the cell wall that may play a role during human fungal infections. Copyright © 2007 John Wiley & Sons, Ltd.