Efficient production of L-lactic acid by Crabtree-negative yeast Candida boidinii
Article first published online: 3 AUG 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Volume 26, Issue 9, pages 485–496, September 2009
How to Cite
Osawa, F., Fujii, T., Nishida, T., Tada, N., Ohnishi, T., Kobayashi, O., Komeda, T. and Yoshida, S. (2009), Efficient production of L-lactic acid by Crabtree-negative yeast Candida boidinii. Yeast, 26: 485–496. doi: 10.1002/yea.1702
- Issue published online: 25 AUG 2009
- Article first published online: 3 AUG 2009
- Manuscript Accepted: 23 JUN 2009
- Manuscript Received: 17 FEB 2009
- Candida boidinii;
- L-lactic acid;
- L-lactate dehydrogenase
Industrial production of L-lactic acid, which in polymerized form as poly-lactic acid is widely used as a biodegradable plastic, has been attracting world-wide attention. By genetic engineering we constructed a strain of the Crabtree-negative yeast Candida boidinii that efficiently produced a large amount of L-lactic acid. The alcohol fermentation pathway of C. boidinii was altered by disruption of the PDC1 gene encoding pyruvate decarboxylase, resulting in an ethanol production that was reduced to 17% of the wild-type strain. The alcohol fermentation pathway of the PDC1 deletion strain was then successfully utilized for the synthesis of L-lactic acid by placing the bovine L-lactate dehydrogenase-encoding gene under the control of the PDC1 promoter by targeted integration. Optimizing the conditions for batch culture in a 5 l jar-fermenter resulted in an L-lactic acid production reaching 85.9 g/l within 48 h. This productivity (1.79 g/l/h) is the highest thus far reported for L-lactic acid-producing yeasts. DDBJ/EMBL/GenBank nucleotide database with Accession Nos. AB440630 and AB440631. Copyright © 2009 John Wiley & Sons, Ltd.