A systematic study of the cell wall composition of Kluyveromyces lactis
Article first published online: 6 MAY 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Special Issue: Special Issue of Yeast on Fungal Cell Walls
Volume 27, Issue 8, pages 647–660, August 2010
How to Cite
Backhaus, K., Heilmann, C. J., Sorgo, A. G., Purschke, G., de Koster, C. G., Klis, F. M. and Heinisch, J. J. (2010), A systematic study of the cell wall composition of Kluyveromyces lactis. Yeast, 27: 647–660. doi: 10.1002/yea.1781
- Issue published online: 3 AUG 2010
- Article first published online: 6 MAY 2010
- Manuscript Accepted: 29 MAR 2010
- Manuscript Received: 8 MAR 2010
- EU Programme. Grant Number: FP7-214004-2 FINSysB
- Deutsche Forschungsgemeinschaft. Grant Number: SFB 431
- cell wall proteins;
In many ascomycetous yeasts, the cell wall is composed of two main types of macromolecules: (a) polysaccharides, with a high content of β-1,6- and β-1,3-linked glucan chains and minor amounts of chitin; and (b) cell wall proteins of different types. Synthesis and maintenance of these macromolecules respond to environmental changes, which are sensed by the cell wall integrity (CWI) signal transduction pathway. We here present a first systematic analysis of the cell wall composition of the milk yeast, Kluyveromyces lactis. Electron microscopic analyses revealed that exponentially growing cells of K. lactis supplied with glucose as a carbon source have a wall thickness of 64 nm, as compared to 105 nm when growing on 3% ethanol. Despite their increased wall thickness, ethanol-grown cells were more sensitive to the presence of zymolyase in the growth medium. Mass spectrometric analysis identified 22 covalently linked cell wall proteins, including 19 GPI-modified proteins and two Pir wall proteins. Importantly, the composition of the cell wall glycoproteome depended on carbon source and growth phase. Our results clearly illustrate the dynamic nature of the cell wall of K. lactis and provide a firm base for studying its regulation. Copyright © 2010 John Wiley & Sons, Ltd.