Ether–zymolyase ascospore isolation procedure: an efficient protocol for ascospores isolation in Saccharomyces cerevisiae yeast
Article first published online: 14 JUL 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Volume 27, Issue 12, pages 999–1003, December 2010
How to Cite
Bahalul, M., Kaneti, G. and Kashi, Y. (2010), Ether–zymolyase ascospore isolation procedure: an efficient protocol for ascospores isolation in Saccharomyces cerevisiae yeast. Yeast, 27: 999–1003. doi: 10.1002/yea.1808
- Issue published online: 1 DEC 2010
- Article first published online: 14 JUL 2010
- Manuscript Accepted: 10 JUN 2010
- Manuscript Received: 12 OCT 2009
- Saccharomyces cerevisiae;
Here we describe a new procedure for ascospore isolation from cultures containing a majority of unsporulated vegetative cells of Saccharomyces cerevisiae. The EZ ascospore isolation procedure relies on the combination of two conventional protocols, diethyl ether treatment and modified zymolyase treatment, allowing a significant increase in the efficiency of ascospore isolation and consequently enabling a large number of meiotic offspring to be efficiently obtained and screened, thus improving the efficacy of genetic research and the genetic selection of S. cerevisiae strains. Copyright © 2010 John Wiley & Sons, Ltd.