Exoglucanase-encoding genes from three Wickerhamomyces anomalus killer strains isolated from olive brine

Authors

  • Serena Muccilli,

    1. DISPA, Sezione di Tecnologia e Microbiologia degli Alimenti, University of Catania, Italy
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    • #These authors contributed equally to this study.
  • Sabrina Wemhoff,

    1. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany
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    • #These authors contributed equally to this study.
  • Cristina Restuccia,

    1. DISPA, Sezione di Tecnologia e Microbiologia degli Alimenti, University of Catania, Italy
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  • Friedhelm Meinhardt

    Corresponding author
    • Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany
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F. Meinhardt. Westfälische Wilhelms-Universität Münster, Institut für Molekulare Mikrobiologie und Biotechnologie, Corrensstrasse 3, 48149 Münster, Germany.

E-mail: meinhar@uni-muenster.de

Abstract

Wickerhamomyces anomalus killer strains are important for fighting pathogenic yeasts and for controlling harmful yeasts and bacteria in the food industry. Targeted disruption of key genes in β-glucan synthesis of a sensitive Saccharomyces cerevisiae strain conferred resistance to the toxins of W. anomalus strains BS91, BCA15 and BCU24 isolated from olive brine. Competitive inhibition of the killing activities by laminarin and pustulan refer to β-1,3- and β-1,6-glucans as the main primary toxin targets. The extracellular exoglucanase-encoding genes WaEXG1 and WaEXG2 from the three strains were sequenced and were found to display noticeable similarities to those from known potent W. anomalus killer strains. Accession Nos for WaEXG1 genes for the strains in brackets are JQ734563 (BS91), JQ734564 (BCA15) and JQ734565 (BCU24); for WaEXG2 genes JQ734566 (BS91), JQ734567 (BCA15) and JQ734568 (BCU24), respectively. Copyright © 2012 John Wiley & Sons, Ltd.

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