The influence of nitrate on the physiology of the yeast Dekkera bruxellensis grown under oxygen limitation

Authors

  • Will de Barros Pita,

    Corresponding author
    • Interdepartmental Research Group on Metabolic Engineering, Federal University of Pernambuco, Recife, PE, Brazil
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  • Ievgeniia Tiukova,

    1. Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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  • Fernanda Cristina Bezerra Leite,

    1. Interdepartmental Research Group on Metabolic Engineering, Federal University of Pernambuco, Recife, PE, Brazil
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  • Volkmar Passoth,

    1. Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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  • Diogo Ardaillon Simões,

    1. Interdepartmental Research Group on Metabolic Engineering, Federal University of Pernambuco, Recife, PE, Brazil
    2. Department of Biochemistry, Federal University of Pernambuco, Recife, PE, Brazil
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  • Marcos Antonio de Morais Jr

    1. Interdepartmental Research Group on Metabolic Engineering, Federal University of Pernambuco, Recife, PE, Brazil
    2. Department of Genetics, Federal University of Pernambuco, Recife, PE, Brazil
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Correspondence to: W. de Barros Pita, Departamento de Genética, Universidade Federal de Pernambuco, Av. Moraes Rego, 1235 Cidade Universitária, 50.670-901 Recife, PE, Brasil.

E-mail: will.barros.pita@gmail.com; Website: www.ufpe.br/nem

Abstract

A previous study showed that the use of nitrate by Dekkera bruxellensis might be an advantageous trait when ammonium is limited in sugarcane substrate for ethanol fermentation. The aim of the present work was to evaluate the influence of nitrate on the yeast physiology during cell growth in different carbon sources under oxygen limitation. If nitrate was the sole source of nitrogen, D. bruxellensis cells presented slower growth, diminished sugar consumption and growth-associated ethanol production, when compared to ammonium. These results were corroborated by the increased expression of genes involved in the pentose phosphate (PP) pathway, the tricarboxylic acid (TCA) cycle and ATP synthesis. The presence of ammonium in the mixed medium restored most parameters to the standard conditions. This work may open up a line of investigation to establish the connection between nitrate assimilation and energetic metabolism in D. bruxellensis and their influence on its fermentative capacity in oxygen-limited or oxygen-depleted conditions. Copyright © 2013 John Wiley & Sons, Ltd.

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