These authors contributed equally to this study.
Quantitative analysis of human ras localization and function in the fission yeast Schizosaccharomyces pombe
Article first published online: 20 MAR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 30, Issue 4, pages 145–156, April 2013
How to Cite
Bond, M., Croft, W., Tyson, R., Bretschneider, T., Davey, J. and Ladds, G. (2013), Quantitative analysis of human ras localization and function in the fission yeast Schizosaccharomyces pombe. Yeast, 30: 145–156. doi: 10.1002/yea.2949
- Issue published online: 12 APR 2013
- Article first published online: 20 MAR 2013
- Accepted manuscript online: 28 FEB 2013 03:41AM EST
- Manuscript Accepted: 12 FEB 2013
- Manuscript Revised: 6 FEB 2013
- Manuscript Received: 8 DEC 2012
- cell signalling;
- Schizosaccharomyces pombe;
- quantitative imaging
Ras signalling is central to fundamental and diverse cellular processes. In higher eukaryotes ras signalling is highly complex, involving multiple isoforms, regulatory proteins and effectors. As a consequence, the study of ras activity in mammalian systems presents a number of technical challenges. The model organism Schizosaccharomyces pombe has previously proved a key system for the study of human signalling components and provides an ideal model for the study of ras, as it contains just one ras protein (Ras1p), which is non-essential and controls a number of downstream processes. Here we present data demonstrating the quantitative analysis of three distinct Ras1-related signalling outputs, utilizing the three most abundant human ras isoforms, H-Ras, N-Ras and K-Ras4B, in Sz. pombe. Further, we have characterized the localization of these three human ras isoforms in Sz. pombe, utilizing quantitative image analysis techniques. These data indicate that all three human ras isoforms are functional in fission yeast, displaying differing localization patterns which correlate strongly with function in the regulation of pheromone response and cell shape. These data demonstrate that such yeast strains could provide powerful tools for the investigation of ras biology, and potentially in the development of cancer therapies. Copyright © 2013 John Wiley & Sons, Ltd.