Present address: Biomedical Research Centre (INIBIC), CH Universitario A Coruña, 15006 A Coruña, Spain
Stalled RNAP-II molecules bound to non-coding rDNA spacers are required for normal nucleolus architecture
Article first published online: 27 JUN 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 30, Issue 7, pages 267–277, July 2013
How to Cite
Freire-Picos, M. A., Landeira-Ameijeiras, V. and Mayán, M. D. (2013), Stalled RNAP-II molecules bound to non-coding rDNA spacers are required for normal nucleolus architecture. Yeast, 30: 267–277. doi: 10.1002/yea.2961
- Issue published online: 9 JUL 2013
- Article first published online: 27 JUN 2013
- Accepted manuscript online: 23 MAY 2013 05:08PM EST
- Manuscript Accepted: 19 MAY 2013
- Manuscript Revised: 16 MAY 2013
- Manuscript Received: 25 FEB 2013
- non-coding regions;
The correct distribution of nuclear domains is critical for the maintenance of normal cellular processes such as transcription and replication, which are regulated depending on their location and surroundings. The most well-characterized nuclear domain, the nucleolus, is essential for cell survival and metabolism. Alterations in nucleolar structure affect nuclear dynamics; however, how the nucleolus and the rest of the nuclear domains are interconnected is largely unknown. In this report, we demonstrate that RNAP-II is vital for the maintenance of the typical crescent-shaped structure of the nucleolar rDNA repeats and rRNA transcription. When stalled RNAP-II molecules are not bound to the chromatin, the nucleolus loses its typical crescent-shaped structure. However, the RNAP-II interaction with Seh1p, or cryptic transcription by RNAP-II, is not critical for morphological changes. Copyright © 2013 John Wiley & Sons, Ltd.