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FilenameFormatSizeDescription
yea_2964_sm_S1_Sugar_mills_localization.docWord document655KSupporting information S1. (A) Map of Brazil, showing the states from which the samples came: Pernambuco (PE), Alagoas (AL) and São Paulo (SP). (B) Map of São Paulo state, showing the area where the plants were located. (C) São Paulo State plants (sugar mills) where the samples were collected: EQ, Equipav; SA, Santa Adélia; MA, Maringá; SE, Serra (CTC); SJ, São João; NS, Nossa Senhora Aparecida; SM, São Manoel; RP, Rio Pardo; MU, Muller Industry (for details, see: https://maps.google.com.br/maps/ms?msid=200944452429190700824.00049db901fb869d5a 538&msa = 0&ll = -22.705255,-47.999268&spn = 4.995393,9.459229). Distances between plants (miles): São Manoel and Rio Pardo, 28.34; São Manuel and Santa Adélia, 95.31; Rio Pardo and Equipav, 106.01; São Manoel and São João, 81.11; São João and Nossa Senhora Aparecida, 31.14; Nossa Senhora Aparecida and Muller Industry, 47.78; Muller Industry and Serra, 36.85; Serra and Maringá, 17.13; Maringá and Santa Adélia, 34.86; Santa Adélia and Equipav, 98.86
yea_2964_sm_S2_table_colony_morphology.docWord document78KSupporting information S2. Morphological aspect of the native and commercial strain colonies grown on WL nutrient medium
yea_2964_sm_8.S3_Loci_location_primer_sequences.docWord document43KSupporting information S3. Location of microsatellite loci on Saccharomyces cerevisiae chromosomes, their respective open reading frames (ORFs) and their respective forward and reverse primer sequences. *Bibliographic references on which the respective primer designs were based
yea_2964_sm_S4a_Capillar_electrophoretic_PCR_amplification.docWord document1565KSupporting information S4. (a) Capillary electrophoretic PCR amplification profile of the commercial strains BG-1, CAT-1, PE-2 and SA-1 by 11 microsatellite markers. (Panels 1–11) Loci G1, G2, G3, G4, G5, G6, G7, G8, G9, G10 and G11, respectively. Virtual gel generated by Qiaxcel Screengel software. The green lines refer to QX Alignment Marker (15 bp/5000 bp for lower and higher bands, respectively). Lines on the right and left refer to QX DNA Size Marker (100 bp–3 kb), which was used to determine the length of the products. (b) Capillary electrophoretic PCR amplification profile of native (1–86; see Table 1) and commercial (BG-1, CAT-1, PE-2 and SA-1) strains by 11 microsatellite markers; panels 1a–c, 2a–d, 3a–c, 4a–c refer to loci G1, G9, G6 and G11, respectively. Virtual gel generated by Qiaxcel Screengel software. The green lines refer to QX Alignment Marker (15/5000 bp for lower and higher bands, respectively). Lines on the right and left refer to QX DNA Size Marker (100 bp–3 kb), which was used to determine the length of the products
yea_2964_sm_S4b_Native_PCR_amplifications.docWord document1721KCapillary electrophoretic PCR amplification profile of native (1 to 86-see Table 1) and commercial strains BG-1, CAT-1, PE-2 and SA-1 by 11 microsatellite markers. Panels 1a to 1c; 2a to 2d; 3a to 3c and 4a to 4c refer to loci G1, G9, G6 and G11 respectively. Virtual gel generated by Qiaxcel Screengel software. The green lines refer to QX Alignment Marker 15bp /5000 bp for lower and higher bands respectively. Lines on the right and left refer to QX DNA Size Marker 100 bp - 3 kb which was used to determine the length of the products.
yea_2964_sm_S5_number_of_heterozygote_per_locus.docWord document187KSupporting information S5. Heterozygotic loci per strain (highlighted in grey)
yea_2964_sm_S6_K_values.docWord document107KSupporting information S6. (Top) Average values of Ln P(D) obtained for each one of the five runs performed for each K value (1–14). Analysis of 11 microsatellite markers on 65 previously assigned strains in seven populations. The most likely K value (K = 6) is highlighted. (Bottom) Graph of log-likelihood Ln P(D) as a K function. Likelihood data shown correspond to the average among five Ln P(D) replication values obtained for each K (1–14)
yea_2964_sm_S7_Structure_K2_to_K6.docWord document307KSupporting information S7. Population structure analysis of 65 S. cerevisiae autochthonous strains. Cluster ranges 2–6 (K = 2–6) resulted from the genetic structure analysis of 11 microsatellite loci amplified in 65 S. cerevisiae yeast strains. Each colour indicates a cluster based on genotypic similarities. Each number (1–7) corresponds to a given sugar mill population: 1, Rio Pardo 2010; 2, São Manoel 2008; 3, São Manoel 2009; 4, Santa Adélia 2009; 5, Santa Adélia 2010; 6, Rio Pardo 2011; 7, strains isolated in Pernambuco

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