In vivo instability of chorismate causes substrate loss during fermentative production of aromatics
Article first published online: 9 JUL 2014
Copyright © 2014 John Wiley & Sons, Ltd.
Volume 31, Issue 9, pages 333–341, September 2014
How to Cite
2014), In vivo instability of chorismate causes substrate loss during fermentative production of aromatics, Yeast, 31, pages 333–341, doi: 10.1002/yea.3025, , and (
- Issue published online: 5 SEP 2014
- Article first published online: 9 JUL 2014
- Accepted manuscript online: 30 JUN 2014 11:11PM EST
- Manuscript Accepted: 9 JUN 2014
- Manuscript Revised: 18 MAY 2014
- Manuscript Received: 6 JAN 2014
- Australian Research Council. Grant Number: DE120101549
- paraaminobezoic acid;
- parahydroxybenzoic acid;
Metabolic engineering of microbial strains to produce aromatic compounds deriving from the shikimate pathway is of great interest to the chemical industry as a more sustainable alternative for feedstock production. Chorismate is a significant intermediate in the shikimate pathway. In this study, the formation of phenylalanine and phenylpyruvate as by-products in strains engineered downstream of the chorismate node for increased aromatic production was explored in yeast fermentations. Tracer experiments showed that these compounds are synthesized de novo during fermentation, under conditions in which their synthesis was genetically blocked. Chorismate stability evaluation, as well as deletion mutation analysis throughout the phenylalanine biosynthesis pathway, suggested that this synthesis was a result of intracellular, non-enzymatic rearrangement of chorismate to phenylpyruvate via prephenate, which was followed by enzymatic transamination of phenylpyruvate to form phenylalanine. These results not only aid in the development of strain-engineering strategies to avoid the accumulation of by-products during fermentations aimed at increased aromatics production, but also deepen our understanding of yeast metabolism. Copyright © 2014 John Wiley & Sons, Ltd.