A comparative radiorespirometric study of glucose metabolism in yeasts

Authors

  • Peter M. Bruinenberg,

    1. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands
    Current affiliation:
    1. AVEBE, Klaas Nieboerweg 12, NL-9607 PN Foxhol, The Netherlands
    Search for more papers by this author
  • Gerrit W. Waslander,

    1. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands
    Search for more papers by this author
  • Johannes P. Van Dijken,

    1. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands
    Search for more papers by this author
  • W. Alexander Scheffers

    Corresponding author
    1. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands
    • Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands
    Search for more papers by this author

Abstract

A comparative radiorespirometric study of glucos emetabolism in glucose-limited chemostat cultures of Saccharomyces cerevisiae, Candida utilis and Rhodosporidium toruloides was performed in an attempt to estimate the contribution of the hexose monophosphate (HMP) pathway to glucose metabolism. Radioactively labelled glucose was administered directly to the cultures in a constant substrate feed, without disturbance of the steady state. The 14CO2 yields from [1-14C]- and [6-14C]-glucose demonstrated that the HMP pathway activities for the three yeasts were very similar. Furthermore, a quantitative analysis of results indicated that the HMP pathway activities were close to the theoretical minimum needed to cover the NADPH requirement for biomass formation.

Ancillary