Cyclic AMP controls the switch between division cycle and resting state programs in response to ammonium availability in Saccharomyces cerevisiae
Article first published online: 29 JAN 2004
Copyright © 1987 John Wiley & Sons Ltd.
Volume 3, Issue 2, pages 85–93, June 1987
How to Cite
Boy-Marcotte, E., Garreau, H. and Jacquet, M. (1987), Cyclic AMP controls the switch between division cycle and resting state programs in response to ammonium availability in Saccharomyces cerevisiae. Yeast, 3: 85–93. doi: 10.1002/yea.320030205
- Issue published online: 29 JAN 2004
- Article first published online: 29 JAN 2004
- Manuscript Revised: 23 DEC 1986
- Manuscript Received: 7 AUG 1986
- Cyclic AMP;
- nitrogen limitation;
- resting state;
- cell cycle
We have identified a mutation called rcal (for rescue by cAMP) which allows adenylate cyclase-deficient mutants to divide in the presence of cAMP. We took advantage of this rcal mutation to study the effect of externally added cAMP on the onset of the resting state when cells are starved for ammonium. We measured the resistance of the cells to zymolyase treatment as a parameter of the resting state. We observed that the onset of the resting state is reversibly blocked by cAMP. This inhibitory effect of cAMP is discussed together with the cAMP control of the start. This leads us to propose a model in which the cAMP level, controlled by the availability of nutrients, should trigger the choice between the entry of the cell into the resting state and the initiation of a new division cycle.