A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors

Authors

  • Nathalie Bonneaud,

    1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
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  • Odile Ozier-Kalogeropoulos,

    1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
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  • Guoya Li,

    1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
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  • Michel Labouesse,

    1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
    Current affiliation:
    1. Department of Biology M.I.T., 77 Massachusetts Avenue, Cambridge, MA 02139, U.S.A
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  • Lionel Minvielle-Sebastia,

    1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
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  • Francois Lacroute

    Corresponding author
    1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
    • Centre de Génétique Moléculaire du C. N. R. S., Laboratoire propre associé á l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, Cedex, France
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Abstract

We describe a set of replicative, integrative and single-stranded shuttle vectors constructed from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast selectable marker: URA3, TRP1 or LEU2. Replicative vectors carrying different yeast replication origins have been constructed in order to have plasmids based on the same construction with a high or low copy number per cell and with different mitotic stabilities. All the vectors are small in size, provide a high yield in Escherichia coli and efficiently transform Saccharomyces cerevisiae. These plasmids have many of the unique sites of the pUC19 multicloning region and many of them allow for the screening of plasmids with an insert by alpha-complementation. The nucleotide sequence of each of them is completely known.

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