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Yeast Sequencing Report
The MAT locus revisited within a 9·8 kb fragment of chromosome III containing BUD5 and two new open reading frames
Article first published online: 29 JAN 2004
DOI: 10.1002/yea.320070815
Copyright © 1991 John Wiley & Sons Ltd.
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Additional Information
How to Cite
Jacquet, M., Buhler, J. M., Iborra, F., Francingues-Gaillard, M. C. and Soustelle, C. (1991), The MAT locus revisited within a 9·8 kb fragment of chromosome III containing BUD5 and two new open reading frames. Yeast, 7: 881–888. doi: 10.1002/yea.320070815
Publication History
- Issue published online: 29 JAN 2004
- Article first published online: 29 JAN 2004
- Manuscript Revised: 25 JUN 1991
- Manuscript Received: 30 MAY 1991
- Abstract
- References
- Cited By
Keywords:
- Chromosome III;
- Saccharomyces cerevisiae;
- mating type;
- HML;
- BUD5
Abstract
This paper reports the DNA sequence of a segment of 9·8 kb of the chromosome III. The sequenced DNA contains the MATα locus. The new sequence of the MATα locus differs from the previously reported sequence by six modifications in the W segment. We have found the same modifications in the HML locus. The corrected sequence contains, in HML, an open reading frame (ORF) of 190 codons which ends at the border between the W segment and the flanking DNA. In the MAT locus, this ORF extends in the flanking DNA up to 538 codons. This ORF corresponds to a gene independently identified as BUD5 (Chant et al., 1991). This gene presents homologies with the exchange factors SDC25 and CDC25. A large ORF of 1399 codons is found on the opposite side of MATα (toward the telomere). This ORF corresponds to a new gene YCR724. Next to this gene is a small ORF, YCR725, of 127 codons. The localization of this fragment on chromosome III, originally supposed to be distal from the MAT locus based on genetic distance, illustrates variation in recombination frequency along the chromosome and suggests the existence of hot spots of recombination between MAT and the THR4 locus.