Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3)

Authors

  • Manuel Simon,

    1. Institut für Allgemeine Biochemie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, A-1090 Vienna, Austria
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  • Maximilian Binder,

    1. Institut für Tumorbiologie-Krebsforschung der Universität Wien, A-1090 Vienna, Austria
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  • Gerhard Adam,

    1. Institut für Allgemeine Biochemie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, A-1090 Vienna, Austria
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  • Andreas Hartig,

    1. Institut für Allgemeine Biochemie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, A-1090 Vienna, Austria
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  • Helmut Ruis

    Corresponding author
    1. Institut für Allgemeine Biochemie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, A-1090 Vienna, Austria
    • Institut für Allgemeine Biochemie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, A-1090 Vienna, Austria
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  • Dedicated to Profesor Wilhelm Fleischhacker on the occasion of his 60th birthday.

Abstract

The Saccharomyces cerevisiae ADR1 gene has recently been demonstrated to control transcription of several genes encoding peroxisomal proteins or proteins necessary for peroxisome formation. Therefore, the effect of two other genes (SNF1 (CAT1, CCR1) and SNF4 (CAT3)) known to control derepression of glucose-repressible genes was studied. Levels of transcripts of genes encoding catalase A, fatty acid β-oxidation enzymes and of the PAS1 gene are reduced in snf1 and snf4 mutants of ethanol as well as on oleic acid medium. By immunogold labelling with an antibody directed against peroxisomal thiolase, clusters of peroxisomes were detected in wild-types cells, whereas smaller single peroxisomes were observed in adr1 mutant cells. Results of immunofluorescence experiments are consistent with these observations. No peroxisomes were detected in snf1 and snf4 mutants by immunogold labelling as well as by imunofluorescence.

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