Yeast Sequencing Report

Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae

  1. Vincenzo Rocco1,
  2. Michael J. Daly2,
  3. Vilborg Matre1,
  4. Michael Lichten2,
  5. Alain Nicolas1,*

Article first published online: 29 JAN 2004

DOI: 10.1002/yea.320091012

Issue

Yeast

Yeast

Volume 9, Issue 10, pages 1111–1120, October 1993

Additional Information

How to Cite

Rocco, V., Daly, M. J., Matre, V., Lichten, M. and Nicolas, A. (1993), Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae. Yeast, 9: 1111–1120. doi: 10.1002/yea.320091012

Author Information

  1. 1

    Institut de Génétique et Microbiologie, CNRS URA 1354, Bât. 4000, Université Paris-Sud, 91405 Orsay cedex, France

  2. 2

    National Cancer Institute, Laboratory of Biochemistry, DCBDC, Building 37, Room 4D14, NIH Bethesda, MD 20892, U.S.A.

*Institut de Génétique et Microbiologie, CNRS URA 1354, Bâ t. 4000, Université Paris-Sud, 91405 Orsay cedex, France

Publication History

  1. Issue published online: 29 JAN 2004
  2. Article first published online: 29 JAN 2004
  3. Manuscript Accepted: 5 MAY 1993
  4. Manuscript Received: 12 MAR 1993

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Keywords:

  • Yeast;
  • Saccharomyces cerevisiae;
  • chromosome VIII;
  • ARG4;
  • meiosis;
  • SH3 domain

Abstract

We have sequenced a 3296 bp segment of the chromosome VIII adjacent to the 3′ end of the ARG4 gene. This segment contains two divergently oriented open reading frames (YSC83 and YSC84). Northern blot analysis showed the presence of transcripts corresponding to these two open reading frames in vegetative cells. Levels of these transcripts increase five to ten-fold during sporulation. These two genes are not essential for vegetative growth or sporulation. Analysis of the putative protein products on the SwissProt database revealed that the C-terminal region of the Ysc84 protein contains a putative SH3 domain.

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