Yeast Sequencing Report
GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae
Article first published online: 29 JAN 2004
Copyright © 1993 John Wiley & Sons Ltd.
Volume 9, Issue 10, pages 1121–1130, October 1993
How to Cite
Rønnow, B. and Kielland-Brandt, M. C. (1993), GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae. Yeast, 9: 1121–1130. doi: 10.1002/yea.320091013
- Issue published online: 29 JAN 2004
- Article first published online: 29 JAN 2004
- Manuscript Accepted: 5 MAY 1993
- Manuscript Received: 20 MAR 1993
- Mitochondrial glycerol 3-phosphate dehydrogenase;
- glycerol utilization;
- Saccharomyces cerevisiae
A gut2 mutant of Saccharomyces cerevisiae is deficient in the mitochondrial glycerol 3-phosphate dehydrogenase and hence cannot utilize glycerol. Upon transformation of a gut2 mutant strain with a low-copy yeast genomic library, hybrid plasmids were isolated which complemented the gut2 mutation. The nucleotide sequence of a 3·2 kb PstI-XhoI fragment complementing a gut2 mutant strain is presented. The fragment reveals an open reading frame (ORF) encoding a polypeptide with a predicted molecular weight of 68·8 kDa. Disruption of the ORF leads to a glycerol non-utilizing phenotype. A putative flavin-binding domain, located at the amino terminus, was identified by comparison with the amino acid sequences of other flavoproteins. The cloned gene has been mapped both physically and genetically to the left arm of chromosome IX, where the original gut2 mutation also maps. We conclude that the presented ORF is the GUT2 gene and propose that it is the structural gene for the mitochondrial glycerol 3-phosphate dehydrogenase.